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PXD022088

PXD022088 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTINC - a method to dissect transcriptional complexes at single locus resolution - reveals novel Nanog regulators in mouse embryonic stem cells
DescriptionCellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in genome-wide epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remains difficult to dissect the majority of factors that interact with these REs due to the lack of appropriate techniques. To address this issue, we developed a novel epigenetic method termed TINC: TALE-mediated Isolation of Nuclear Chromatin. Using TINC we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known interactors as well as numerous previously unknown complex members, including RCOR2. Further interrogation of the role of RCOR2 in the ESC network revealed its involvement in the repression of lineage genes and the fine-tuning of pluripotency genes. Consequently, using the Nanog promoter as a paradigm, we demonstrated the power of TINC to provide important insights into the molecular makeup of transcriptional complexes at individual REs as well as into control of cellular identity in general.
HostingRepositoryPRIDE
AnnounceDate2020-12-14
AnnouncementXMLSubmission_2020-12-14_00:14:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRalf Schittenhelm
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion; QTRAP 6500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-20 01:15:06ID requested
12020-12-14 00:14:14announced
Publication List
Knaupp AS, Mohenska M, Larcombe MR, Ford E, Lim SM, Wong K, Chen J, Firas J, Huang C, Liu X, Nguyen T, Sun YBY, Holmes ML, Tripathi P, Pflueger J, Rossello FJ, Schr, รถ, der J, Davidson KC, Nefzger CM, Das PP, Haigh JJ, Lister R, Schittenhelm RB, Polo JM, TINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter. Stem Cell Reports, 15(6):1246-1259(2020) [pubmed]
Keyword List
submitter keyword: TINC, stem cells, cellular identity, regulatory elements, Nanog
Contact List
Jose Polo
contact affiliationDepartment of Anatomy and Developmental Biology, Australian Regenerative Medicine Institute, Monash University, Australia
contact emailjose.polo@monash.edu
lab head
Ralf Schittenhelm
contact affiliationMonash University
contact emailralf.schittenhelm@monash.edu
dataset submitter
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Dataset FTP location
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