PXD022080

PXD022080 is an original dataset announced via ProteomeXchange.

Title	The MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands
Description	An IP–MS method was used to screen for MbovP280-binding proteins. Briefly, BoMac cells were cultured, harvested, and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). The whole-cell lysates (400 μg) were incubated with 10 μg of either rMbovP280 or rMbovP280∆210–269 for 1 h at 4 C. Mouse antiserum (5 μg) directed against MbovP280 was added to the lysates for 12 h at 4 C, and then 50 μl of Protein A/G Agarose Beads (Beyotime) was added for an additional 1 h. The immunoprecipitates were extensively washed with NP-40 buffer and eluted with SDS loading buffer by boiling them for 5 min. The cellular proteins that coprecipitated with rMbovP280 or rMbovP280∆210–269 were resolved with SDS-PAGE and stained with silver staining.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:34:27.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gang Zhao
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 08:30:47	ID requested
⏵ 1	2021-09-09 10:34:28	announced

Zhao G, Zhu X, Zhang H, Chen Y, Schieck E, Hu C, Chen H, Guo A, MbovP280 Induces Macrophage Apoptosis Through CRYAB. Front Immunol, 12():619362(2021) [pubmed]

submitter keyword: Bovine, BoMac, IP,Mycoplasma bovis, MbovP280,LC-MS/MS

Aizhen Guo
contact affiliation	Huazhong Agricultural University, China
contact email	aizhen@mail.hzau.edu.cn
lab head
Gang Zhao
contact affiliation	Huazhong Agricultural University
contact email	happygang@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022080
     1. Label: PRIDE project
     2. Name: The MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands