PXD022080 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands |
Description | An IP–MS method was used to screen for MbovP280-binding proteins. Briefly, BoMac cells were cultured, harvested, and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). The whole-cell lysates (400 μg) were incubated with 10 μg of either rMbovP280 or rMbovP280∆210–269 for 1 h at 4 C. Mouse antiserum (5 μg) directed against MbovP280 was added to the lysates for 12 h at 4 C, and then 50 μl of Protein A/G Agarose Beads (Beyotime) was added for an additional 1 h. The immunoprecipitates were extensively washed with NP-40 buffer and eluted with SDS loading buffer by boiling them for 5 min. The cellular proteins that coprecipitated with rMbovP280 or rMbovP280∆210–269 were resolved with SDS-PAGE and stained with silver staining. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_10:34:27.379.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gang Zhao |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-19 08:30:47 | ID requested | |
⏵ 1 | 2021-09-09 10:34:28 | announced | |
Publication List
Zhao G, Zhu X, Zhang H, Chen Y, Schieck E, Hu C, Chen H, Guo A, MbovP280 Induces Macrophage Apoptosis Through CRYAB. Front Immunol, 12():619362(2021) [pubmed] |
Keyword List
submitter keyword: Bovine, BoMac, IP,Mycoplasma bovis, MbovP280,LC-MS/MS |
Contact List
Aizhen Guo |
contact affiliation | Huazhong Agricultural University, China |
contact email | aizhen@mail.hzau.edu.cn |
lab head | |
Gang Zhao |
contact affiliation | Huazhong Agricultural University |
contact email | happygang@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022080
- Label: PRIDE project
- Name: The MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands