⮝ Full datasets listing

PXD022080

PXD022080 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe MbovP280 of mycoplasma bovis immunoprecipitates with the wole cell lysates of bovine macrophage (BoMac),and then LC-MS/MS was used to identify the MbovP280-binding ligands
DescriptionAn IP–MS method was used to screen for MbovP280-binding proteins. Briefly, BoMac cells were cultured, harvested, and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). The whole-cell lysates (400 μg) were incubated with 10 μg of either rMbovP280 or rMbovP280∆210–269 for 1 h at 4 C. Mouse antiserum (5 μg) directed against MbovP280 was added to the lysates for 12 h at 4 C, and then 50 μl of Protein A/G Agarose Beads (Beyotime) was added for an additional 1 h. The immunoprecipitates were extensively washed with NP-40 buffer and eluted with SDS loading buffer by boiling them for 5 min. The cellular proteins that coprecipitated with rMbovP280 or rMbovP280∆210–269 were resolved with SDS-PAGE and stained with silver staining.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-09_10:34:27.379.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGang Zhao
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-19 08:30:47ID requested
12021-09-09 10:34:28announced
Publication List
Zhao G, Zhu X, Zhang H, Chen Y, Schieck E, Hu C, Chen H, Guo A, MbovP280 Induces Macrophage Apoptosis Through CRYAB. Front Immunol, 12():619362(2021) [pubmed]
Keyword List
submitter keyword: Bovine, BoMac, IP,Mycoplasma bovis, MbovP280,LC-MS/MS
Contact List
Aizhen Guo
contact affiliationHuazhong Agricultural University, China
contact emailaizhen@mail.hzau.edu.cn
lab head
Gang Zhao
contact affiliationHuazhong Agricultural University
contact emailhappygang@outlook.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022080
PRIDE project URI
Repository Record List
[ + ]