PXD022073

PXD022073 is an original dataset announced via ProteomeXchange.

Title	The Akirin2 regulome and interactome reveal gene/protein targets, candidate functional complements and role in epigenetic regulation
Description	The Akirin (AKR) family of transcription cofactors are involved in the regulation of different biological processes such as immunity, interdigital regression, muscle and neural development throughout the metazoan. This model regulatory proteins do not have catalytic or DNA-binding capability and exert its regulatory function primarily through interacting proteins such as transcription factors, chromatin remodelers, and RNA-associated proteins. The application of regulomics and interactomics to key transcriptional regulators such as AKR is important to better understand their role in cell biology and epigenetic regulation of gene expression. In this study, we focused on the human AKR2 regulome and interactome to advance the knowledge on the function of this highly conserved regulatory cofactor. Based on the key function of AKR2 in cell interactome, regulome and epigenetic gene regulation, herein we hypothesized that metazoan evolved to have AKR2 functional complements and different mechanisms for AKR2-mediated epigenetic regulation of gene expression. To address this hypothesis, experiments were conducted to identify AKR2 gene/protein targets, functional complements after akr2 knockdown in human cells and to provide evidence of different mechanisms that may be involved in AKR2-mediated chromatin remodeling. The results revealed multiple AKR2 gene/protein targets, identified immune response genes as candidate AKR2 functional complements and showed that AKR2 is not only a link with chromatin remodelers, but also directly interacts with histone H3, thus providing new information on the function of this protein
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:39:03.899.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022073
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Margarita Villar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 05:18:14	ID requested
⏵ 1	2021-09-09 10:39:04	announced

Artigas-Jer, ó, nimo S, Villar M, Estrada-Pe, ñ, a A, Vel, á, zquez-Campoy A, Alberdi P, de la Fuente J, Function of cofactor Akirin2 in the regulation of gene expression in model human Caucasian neutrophil-like HL60 cells. Biosci Rep, 41(7):(2021) [pubmed]

submitter keyword: HL60, Akirin2, knockdown, regulome, interactome

Margarita Villar
contact affiliation	1. SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005, Ciudad Real, Spain. 2. Biochemistry Section, Faculty of Science and Chemical Technologies, and Regional Centre for Biomedical Research (CRIB), University of Castilla-La Mancha, 13071, Ciudad Real, Spain.
contact email	margaritam.villar@uclm.es
lab head
Margarita Villar
contact affiliation	University of Castilla- La Mancha
contact email	margaritam.villar@uclm.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022073
     1. Label: PRIDE project
     2. Name: The Akirin2 regulome and interactome reveal gene/protein targets, candidate functional complements and role in epigenetic regulation