PXD022066

PXD022066 is an original dataset announced via ProteomeXchange.

Title	A regulatory role for CHD4 in maintenance of the spermatogonial stem cell pool
Description	Maintenance and self-renewal of the spermatogonial stem cell (SSC) population is the cornerstone of male fertility. In this manuscript we have identified a key role for the nucleosome remodelling protein Chromodomain Helicase DNA binding protein 4 (CHD4) in regulating SSC function. Gene expression analyses revealed that CHD4 expression is largely restricted to spermatogonia in the mouse testis, and is particularly enriched in SSCs. Using spermatogonial transplantation techniques and RNAi mediated knockdown it was established that loss of Chd4 expression significantly impairs SSC regenerative capacity, resulting in a ~50% reduction in colonisation of recipient testes. A single cell RNA-seq comparison depicted reduced expression of ‘self-renewal’ genes such as Gfra1 and Pten following Chd4 knockdown, along with increased expression of signature progenitor genes, Neurog3 and Dazl. Co-immunoprecipitation analyses demonstrated that CHD4 regulates gene expression in spermatogonia not only though its traditional association with the remodelling complex NuRD, but also via interaction with the GDNF-responsive transcription factor SALL4. Cumulatively, the results of this study depict a previously unappreciated fundamental role for CHD4 in controlling fate decisions in the spermatogonial pool.
HostingRepository	PRIDE
AnnounceDate	2021-06-18
AnnouncementXML	Submission_2021-06-18_05:26:55.659.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022066
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 03:54:03	ID requested
⏵ 1	2021-06-18 05:26:56	announced

Cafe SL, Skerrett-Byrne DA, De Oliveira CS, Nixon B, Oatley MJ, Oatley JM, Lord T, A regulatory role for CHD4 in maintenance of the spermatogonial stem cell pool. Stem Cell Reports, 16(6):1555-1567(2021) [pubmed]

submitter keyword: Spermatogonial stem cell, self-renewal, CHD4, Mi-2β

Tessa Lord
contact affiliation	Priority Research Centre for Reproductive Science, Discipline of Biological Sciences, The University of Newcastle, Callaghan, NSW, 2300, Australia; School of Molecular Biosciences, Centre for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164, USA.
contact email	tessa.lord@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	The University of Newcastle
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD022066

PRIDE project URI

• PRIDE
  1. PXD022066
     1. Label: PRIDE project
     2. Name: A regulatory role for CHD4 in maintenance of the spermatogonial stem cell pool