PXD022064

PXD022064 is an original dataset announced via ProteomeXchange.

Title	Defense Response to Caterpillar Feeding Stress in Wild Pinus tabuliformis Unveiled by Quantitative Integrated Proteomic and Phosphoproteomic Analyses
Description	To study the alteration pattern and defensive response mechanism triggered by herbivorous feeding stimuli under natural conditions, we built a biological model of the interrelationship between the Chinese pine (Pinus tabuliformis Carr.) and the Chinese pine caterpillar (Dendrolimus tabulaeformis Tsai et Liu) within their native habitat. We integrated proteomic and phosphoproteomic data, normalized the results, and combined them with bioinformatics to evaluate and analyze variations in phosphoproteomics in pine needles' response to the caterpillar's feeding stimulus. We systematically identified differentially significant phosphorylated proteins implicated in the pine's defense mechanism against caterpillar stress. Furthermore, we predicted upstream kinases of phosphorylation sites and their activities. Similarly, through an analysis of the Motif patterns of phosphorylated proteins, Mfuzz clustering of phosphorylation sites, kinase regulatory networks and functional modules of phosphorylated protein interaction networks in response to stress within pine, we can investigate the mechanisms behind resistance formation and regulation of caterpillar feeding incentives in pine. The identification results of partially phosphorylated proteins were additionally confirmed through PRM technology. Furthermore, genes upstream of differentially expressed proteins were validated through RT-qPCR detection.
HostingRepository	PRIDE
AnnounceDate	2024-01-16
AnnouncementXML	Submission_2024-01-15_19:32:19.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianhua Sun
SpeciesList	scientific name: Pinus fenzeliana; NCBI TaxID: 252739;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 03:53:47	ID requested
⏵ 1	2024-01-15 19:32:19	announced

Dataset with its publication pending

submitter keyword: Chinese pine

Pine caterpillar

Induced resistance

Proteomics

Sun tianhua
contact affiliation	Hebei agricultural university
contact email	tianhua0001@163.com
lab head
Tianhua Sun
contact affiliation	+8615933935320
contact email	tianhua0001@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022064
     1. Label: PRIDE project
     2. Name: Defense Response to Caterpillar Feeding Stress in Wild Pinus tabuliformis Unveiled by Quantitative Integrated Proteomic and Phosphoproteomic Analyses