PXD022044

PXD022044 is an original dataset announced via ProteomeXchange.

Title	Mapping and quantification of phosphorylation sites on protein kinase Akt by LC-MS/MS
Description	Mass spectrometry analysis of various Akt species was a cornerstone for elucidating the mechanism of its activation/autoinhibition. By combining intact mass measurements with LC-MS/MS we were able to characterize Akt species comprehensively and relate their biological activity to specific phosphorylation patterns.
HostingRepository	PRIDE
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-03-20_04:48:43.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DorotheaAnrather
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 08:44:33	ID requested
1	2021-08-13 01:26:17	announced
⏵ 2	2023-03-20 04:48:44	announced	2023-03-20: Updated project metadata.

Truebestein L, Hornegger H, Anrather D, Hartl M, Fleming KD, Stariha JTB, Pardon E, Steyaert J, Burke JE, Leonard TA, -mediated activation. Proc Natl Acad Sci U S A, 118(33):(2021) [pubmed]

submitter keyword: growth factor signaling, PIP3,Akt1, lipid second messengers

Thomas A.Leonard
contact affiliation	Department of Structural and Computational Biology, Max Perutz Labs, Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact email	thomas.leonard@medunivie.ac.at
lab head
DorotheaAnrather
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, University of Vienna
contact email	dorothea.anrather@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD022044

PRIDE project URI

• PRIDE
  1. PXD022044
     1. Label: PRIDE project
     2. Name: Mapping and quantification of phosphorylation sites on protein kinase Akt by LC-MS/MS