PXD022031

PXD022031 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics between Brucella abortus 2308 and Genetically Marked Vaccine A19ΔvirB12 Reveal Attenuation Mechanism and Supplementary Differential Diagnosis Antigens
Description	Brucella abortus is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The B. abortus A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, A19 has the limitation of diagnosis interference and residual virulence for the host. In this study, a novel genetically marked vaccine, A19ΔvirB12, was generated and evaluated. The results indicated that A19ΔvirB12 was able to provide effective protection against B. abortus 2308 (S2308) challenge in mice and vaccinated sera can be distinguished from infected sera. However, previous studies have found that the accuracy of the serological detection method based on VirB12 protein needs to be improved. Therefore, we attempted to identify potential supplementary antigens with differential diagnosis function using label-free quantitative proteomics. Eighteen proteins identified only in S2308 were screened, and 7 of them were predicted to have high probability of antigenicity. In addition, 12 virulence factors were upregulated in S2308. The upregulation pathways of S2308 were significantly enriched in quorum sensing, ATP-binding cassette transporter, and metabolism. Several proteins related to cell division were significantly downregulated, while some proteins involved in transcription were upregulated in S2308.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:27:24.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianghua Yang
SpeciesList	scientific name: Brucella abortus 2308; NCBI TaxID: 359391;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 03:46:25	ID requested
⏵ 1	2021-09-09 10:27:25	announced

Yang J, He C, Zhang H, Liu M, Zhao H, Ren L, Wu D, Du F, Liu B, Han X, He S, Chen Z, virB12 for Cattle. Front Immunol, 12():679560(2021) [pubmed]

submitter keyword: B. abortus

A19ΔvirB12

Proteomics

Differential diagnosis

Attenuation mechanism

zeliang Chen
contact affiliation	Shenyang Agricultural University
contact email	zeliangchen@yahoo.com
lab head
Jianghua Yang
contact affiliation	Shenyang Agricultural University
contact email	Yjh20191207@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022031
     1. Label: PRIDE project
     2. Name: Comparative Proteomics between Brucella abortus 2308 and Genetically Marked Vaccine A19ΔvirB12 Reveal Attenuation Mechanism and Supplementary Differential Diagnosis Antigens