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PXD022028

PXD022028 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHost cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.
DescriptionBCG strain overexpressing the toxin (vapC12)–antitoxin (vapB12)-His(6X) locus was used for sample preparation. Log phase culture of the overexpressed strain was washed with PBS and inoculated in minimal media with 0.1% glycerol and minimal media with 0.01% cholesterol. The cultures were allowed to grow for 48 hrs and cell lysate was prepared. Immunoprecipitation was performed using an anti-His antibody (BTL1010, Biospecs). In-solution digestion was carried out for 10 µg of proteins from each condition. The samples were subjected to reduction and alkylation using 5 mM dithiothreitol (DTT) (60°C for 45 minutes) and alkylation using 10 mM Iodoacetamide (IAA). Trypsin (Gold mass-spectrometry trypsin; Promega, Madison, WI) digestion was carried out at 37°C for 10-12 hrs. The peptides were vacuum-dried and stored at -80°C until LC-MS/MS analysis.
HostingRepositoryPRIDE
AnnounceDate2020-10-16
AnnouncementXMLSubmission_2020-10-16_06:36:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRenu Goel
SpeciesList scientific name: Mycobacterium tuberculosis M24; NCBI TaxID: 1268063;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-16 03:46:13ID requested
12020-10-16 06:36:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, LC-MS/MS
Contact List
Dr Amit Pandey
contact affiliationTranslational Health Science and Technology Institute
contact emailamitpandey@thsti.res.in
lab head
Renu Goel
contact affiliationTranslational Health Science and technology Institute
contact emaildrrenugoel@gmail.com
dataset submitter
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Dataset FTP location
PRIDE project URI
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