PXD022027

PXD022027 is an original dataset announced via ProteomeXchange.

Title	Multi-cell type proteomic profiling reveals desensitization of SARS-CoV-2-infected cells to interferon treatment
Description	SARS-CoV-2, the coronavirus behind the ongoing pandemic, manifests itself in a broad array of symptoms involving various body organs, such as lungs, intestine, kidneys, heart, liver, and brain. To investigate how SARS-CoV-2 navigates disparate organs and alters their biology, we engineered a panel of phenotypically diverse human cell lines representing different organs that support efficient virus infection. We leveraged these infection models to profile tissue-specific host responses to SARS-CoV-2 infection by global proteomic analyses. This uncovered broad as well as cell type-specific perturbations of cellular proteins, several of which we subsequently validated by an orthogonal approach. Our detailed follow-up investigation of a number of proteins in different cell types, including a stem cell-derived model of virus infection, revealed almost complete desensitization of SARS-CoV-2-infected cells to interferon treatment. These findings elucidate the immune evasion mechanisms of SARS-CoV-2 and have implications for the currently evaluated antiviral regimens involving interferon (148).
HostingRepository	PRIDE
AnnounceDate	2021-07-27
AnnouncementXML	Submission_2021-07-27_14:31:40.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Heckendorf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 01:40:48	ID requested
⏵ 1	2021-07-27 14:31:40	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, human cell lines, proteomics, virus-host interactions, interferon signaling, immune evasion, receptor tyrosine kinases, STAT phosphorylation

Mohsan Saeed
contact affiliation	Assistant Professor, Biochemistry Boston University School of Medicine Investigator, National Emerging Infectious Diseases Laboratories (NEIDL), USA
contact email	msaeed1@bu.edu
lab head
Christian Heckendorf
contact affiliation	Boston University
contact email	heckend@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD022027

PRIDE project URI

• PRIDE
  1. PXD022027
     1. Label: PRIDE project
     2. Name: Multi-cell type proteomic profiling reveals desensitization of SARS-CoV-2-infected cells to interferon treatment