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PXD022019 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe influence of delay in mononuclear cell isolation on acute myeloid leukemia phosphorylation profiles
DescriptionPhosphorylation is a dynamic, highly regulated post-translational modification that may be affected by variation in pre-analytical sample handling, hampering the translational value of phosphoproteomic-based data. Here, we investigate the temporal effect of delay in mononuclear cell isolation on acute myeloid leukemia (AML) phosphorylation profiles. We performed MS on immuno-precipitated phosphotyrosine (pY)-containing peptides isolated from AML samples after seven pre-defined delays before sample processing (direct processing, thirty minutes, one hour, two hours, three hours, four hours and 24 hours delay). Up to four hours, phosphorylation profiles showed limited variation. However, in samples processed with a delay of 24 hours, we observed significant change in phosphorylation profiles, with differential phosphorylation of 22 phosphopeptides (p < 0.01). This included increased phosphorylation of phosphopeptides of JNK and p38 kinases indicative of stress response activation. Based on these results, we conclude that processing of AML samples should be standardized at all times and should occur within four hours after sample collection.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSander Piersma
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-16 01:25:24ID requested
12021-02-22 05:57:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Acute myeloid leukemia, AML, phosphoproteomics, phosphotyrosine, ischemia, oxidative stress, pre-analytical variables, tyrosine kinase
Contact List
Connie Ramona Jimenez
contact affiliationOncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
contact emailc.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliationAmsterdam UMC
contact emails.piersma@amsterdamumc.nl
dataset submitter
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