PXD022014

PXD022014 is an original dataset announced via ProteomeXchange.

Title	Proteomic research on effect of EIF4A1 inhibition in MYC-amplified Group 3 medulloblastoma
Description	As the most life-threatening subtype of pediatric medulloblastoma (MB), MYC-amplified Group 3 (G3) MB lacks effective and selective therapeutics. We tried to indirectly target MYC (oncogenic driver and cancer-dependent molecule) production via inhibiting translational machinery. Through multiple datasets analyses on components of eIF4F (eukaryotic translation initiation factor 4F) complex, we found that EIF4A1 (major component with RNA helicase activity) has relatively higher expression level and the closest positive correlation with MYC in G3-MB among normal control and other 3 subtypes (e.g., WNT, SHH and G4). Both in vitro and in vivo experiments with MYC-amplified G3-MB cell lines showed effective growth inhibition upon EIF4A1 knockout or inhibitor treatment. Further FACS analysis found decreased cell proliferation and enhanced apoptosis on EIF4A1 inhibitor treatment. To explore the mechanisms of EIF4A1 inhibition on arresting MYC-amplified G3-MB, we performed the whole proteome analyses on corresponding MB cells treated with EIF4A1 inhibitor.
HostingRepository	iProX
AnnounceDate	2020-10-15
AnnouncementXML	Submission_2021-03-11_00:10:31.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	TMT6plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 19:46:21	ID requested
1	2020-12-03 00:36:05	announced
⏵ 2	2021-03-11 00:10:32	announced	2021-03-11: Update publication information.

Zhao Y, Li T, Tian S, Meng W, Sui Y, Yang J, Wang B, Liang Z, Zhao H, Han Y, Tang Y, Zhang L, Ma J, -Amplified Group 3 Medulloblastoma Through Targeting EIF4A1. Cancer Manag Res, 12():12473-12485(2020) [pubmed]

submitter keyword: proteomic, EIF4A1, medulloblastoma, MYC, Group 3

Yujie Tang
contact affiliation	Shanghai Jiao Tong University, School of Medicine
contact email	yujietang@shsmu.edu.cn
lab head
Lei Zhang
contact affiliation	Shanghai Jiao Tong University, School of Medicine
contact email	lei.zhang2012@sjtu.edu.cn
dataset submitter

iProX dataset URI