PXD022008

PXD022008 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human mammospheres treated with lysine and estradiol
Description	Despite anti-estrogen therapy, almost 30% of estrogen receptor positive (ER+) breast cancer patients relapse. Amino acids (AAs) in the tumor microenvironment may affect the metastatic capacity of breast cancer cells. Essential AAs (EAAs) cannot be produced by human cells and might therefore be targetable as therapeutics. By using liquid chromatography-mass spectrometry we identified the ribophorin-2 (RPN2) and the splicing factor U2AF 35 kDa subunit (U2AF1) as the most significantly affected proteins after lysine and estradiol (E2) exposure in T47D and MCF-7 mammospheres, respectively. RPN2 and U2AF1 were identified as prognostic factors for patient survival in breast cancer databases.
HostingRepository	PRIDE
AnnounceDate	2020-11-25
AnnouncementXML	Submission_2020-11-25_06:47:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annelie Abrahamsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 07:04:15	ID requested
⏵ 1	2020-11-25 06:47:33	announced

Dataset with its publication pending

submitter keyword: LC-MS, breast cancer, essential amino acids, lysine, dissemination

Charlotta Dabrosin
contact affiliation	Linköping University, Sweden
contact email	charlotta.dabrosin@liu.se
lab head
Annelie Abrahamsson
contact affiliation	Linköpings Universitet
contact email	annelie.abrahamsson@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD022008

PRIDE project URI

• PRIDE
  1. PXD022008
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human mammospheres treated with lysine and estradiol