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PXD021996

PXD021996 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA novel structure of GPI anchored proteins
DescriptionGPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. Based on the finding that PIGB-knockout cells lacking the third mannose and PIGO-knockout cells lacking EthN-P linked to the third mannose expressed low levels of CD59 and DAF, GPI-APs and that those residual GPI-APs were lost by further knockout of PIGG. Using mass-spectrometry we determined that CD59 expressed on PIGB-knockout cells is attached to GPI via EthN-P linked to the second mannose. This linkage structure is also found in wild type cells. Our data modifies the current view of GPI anchors and provides mechanistic basis of inherited GPI deficiency caused by PIGG mutations.
HostingRepositoryPRIDE
AnnounceDate2022-05-31
AnnouncementXMLSubmission_2022-05-31_06:09:29.195.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021996
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYoshiko Murakami
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-15 01:16:18ID requested
12022-05-31 06:09:30announced
Publication List
Ishida M, Maki Y, Ninomiya A, Takada Y, Campeau P, Kinoshita T, Murakami Y, Ethanolamine-phosphate on the second mannose is a preferential bridge for some GPI-anchored proteins. EMBO Rep, 23(7):e54352(2022) [pubmed]
Keyword List
submitter keyword: Human, LC-ESI- MS, GPI anchor, PIGG
Contact List
Taroh Kinoshita
contact affiliationRIMD, Osaka university,Japan
contact emailtkinoshi@biken.osaka-u.ac.jp
lab head
Yoshiko Murakami
contact affiliationResearch Institute for Microbial Diseases Osaka University
contact emailyoshiko@biken.osaka-u.ac.jp
dataset submitter
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Dataset FTP location
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