PXD021996

PXD021996 is an original dataset announced via ProteomeXchange.

Title	A novel structure of GPI anchored proteins
Description	GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. Based on the finding that PIGB-knockout cells lacking the third mannose and PIGO-knockout cells lacking EthN-P linked to the third mannose expressed low levels of CD59 and DAF, GPI-APs and that those residual GPI-APs were lost by further knockout of PIGG. Using mass-spectrometry we determined that CD59 expressed on PIGB-knockout cells is attached to GPI via EthN-P linked to the second mannose. This linkage structure is also found in wild type cells. Our data modifies the current view of GPI anchors and provides mechanistic basis of inherited GPI deficiency caused by PIGG mutations.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_06:09:29.195.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021996
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yoshiko Murakami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 01:16:18	ID requested
⏵ 1	2022-05-31 06:09:30	announced

Ishida M, Maki Y, Ninomiya A, Takada Y, Campeau P, Kinoshita T, Murakami Y, Ethanolamine-phosphate on the second mannose is a preferential bridge for some GPI-anchored proteins. EMBO Rep, 23(7):e54352(2022) [pubmed]

submitter keyword: Human, LC-ESI- MS, GPI anchor, PIGG

Taroh Kinoshita
contact affiliation	RIMD, Osaka university,Japan
contact email	tkinoshi@biken.osaka-u.ac.jp
lab head
Yoshiko Murakami
contact affiliation	Research Institute for Microbial Diseases Osaka University
contact email	yoshiko@biken.osaka-u.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021996
     1. Label: PRIDE project
     2. Name: A novel structure of GPI anchored proteins