PXD021994

PXD021994 is an original dataset announced via ProteomeXchange.

Title	Proteomics studies of outer and inner cellular layers of tomato root-tips for Al stress
Description	Excess levels of Al3+ become highly harmful to plant roots. The epidermal and outer cortical layers of root-tips experience more severe damages than the inner tissues from Al toxicity. This project used laser capture microdissection (LCM) technology to isolate the outer and inner cell layers of cells in root-tips. Tomato "MicroTom" plants were grown in hydroponic solution supplemented with Al. Seeds derived from plants previously exposed to four generations of Al-treated solutions were considered stress-acclimated, these seeds were named G4Al+. Seeds harvested from soil-grown plants were also used, these seeds were named G0 seeds. Tandem mass tag (TMT) proteomics was used to identify differential proteomics responses among cells localized at different spaces in root-tips and those between the stress-acclimated and non-acclimated plants.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_02:38:00.209.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021994
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ted Thannhauser
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 01:15:57	ID requested
⏵ 1	2022-02-14 02:38:00	announced

Potts J, Li H, Qin Y, Wu X, Hui D, Nasr KA, Zhou S, Yong Y, Fish T, Liu J, Thannhauser TW, Using single cell type proteomics to identify Al-induced proteomes in outer layer cells and interior tissues in the apical meristem/cell division regions of tomato root-tips. J Proteomics, 255():104486(2022) [pubmed]

submitter keyword: Outer and inner cellular layers of root-tips, Laser Capture Microdissection, TMT proteomics, Aluminum toxicity

Theodore Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
lab head
Ted Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021994

PRIDE project URI

• PRIDE
  1. PXD021994
     1. Label: PRIDE project
     2. Name: Proteomics studies of outer and inner cellular layers of tomato root-tips for Al stress