PXD021982

PXD021982 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Interferon Regulatory Factors: IRF1 and IRF7 directly regulate gene expression in bats in response to viral infection.
Description	Bat cells and tissue have elevated basal expression levels of antiviral genes commonly associated with IFNα signalling. Here we show IRF1,3 and 7 levels are elevated in most bat tissues and that basally, IRFs contribute to the expression of type I IFN ligands and high expression of Interferon Regulated Genes (IRGs). CRISPR KO of IRF 1/3/7 in cells reveal distinct subsets of genes affected by each IRF in an IFN-ligand signalling dependent and largely independent manner. As the master regulators of innate immunity, the IRFs, control the kinetics and maintenance of the IRG response and play essential roles in response to IAV, HSV1, PRV3M and MERS-CoV infection. With its differential expression compared to human, this highlights a critical role for basal IRF expression in viral responses and potentially immune cell development in bats with relevance for IRF function in human biology.
HostingRepository	jPOST
AnnounceDate	2020-10-23
AnnouncementXML	Submission_2023-04-02_20:26:39.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; deamidated L-glutamine; deamidated L-asparagine; TMT6plex reporter+balance reagent acylated N-terminal; TMT6plex reporter+balance reagent N-acylated residue
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-14 22:56:37	ID requested
1	2020-10-23 02:34:58	announced
2	2021-02-04 17:19:18	announced	2021-02-04: Updated FTP location.
3	2022-09-18 03:58:31	announced	2022-09-18: Updated FTP location.
⏵ 4	2023-04-02 20:26:40	announced	2023-04-03: Updated PubMed.

Publication List

Irving AT, Zhang Q, Kong PS, Luko K, Rozario P, Wen M, Zhu F, Zhou P, Ng JHJ, Sobota RM, Wang LF, Interferon Regulatory Factors IRF1 and IRF7 Directly Regulate Gene Expression in Bats in Response to Viral Infection. Cell Rep, 33(5):108345(2020) [pubmed]

Irving AT, Zhang Q, Kong PS, Luko K, Rozario P, Wen M, Zhu F, Zhou P, Ng JHJ, Sobota RM, Wang LF, Interferon Regulatory Factors IRF1 and IRF7 Directly Regulate Gene Expression in Bats in Response to Viral Infection. Cell Rep, 33(5):108345(2020) [pubmed]

Keyword List

submitter keyword: Bat, inflammation, IFN, IRF

submitter keyword: Bat, inflammation, IFN, IRF

Contact List

Dr. Aaron T Irving
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000983/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000983/