PXD021980

PXD021980 is an original dataset announced via ProteomeXchange.

Title	HBXIP-mediated cell cycle, apoptosis and autophagy participate in the inhibitory effect of Germacrone on gastric cancer cells
Description	As a monocyclic sesquiterpene, Germacrone has remarkable anti-tumor effect in a variety of cancers such as hepatocellular carcinoma, gastric cancer, and breast cancer. However, the mechanism of Germacrone on gastric cancer was still unclear. In our study, Germacrone inhibited gastric cancer cells proliferation in a dose-dependent manner. As shown, Germacrone induced G0 / G1 phase arrest and apoptosis in gastric cancer. Germacrone increased the expression of LC3ii/LC3i, p62. Then combined with CQ, the expression of LC3ii/LC3i, p62 continued to increase. As a result, Germacrone partially blocked the autophagy process. Through proteomic technology, a total of 596 proteins were screened and the top hits were Hepatitis B X-interacting protein (HBXIP). Overexpression of HBXIP delayed the cycle arrest, induction of apoptosis, and autophagy inhibition induced by Germacrone. Germacrone inhibits proliferation in gastric cancer cells by inhibiting HBXIP, and this process is related to G0 / G1 phase arrest, induction apoptosis, and autophagy inhibition.
HostingRepository	iProX
AnnounceDate	2020-10-14
AnnouncementXML	Submission_2023-03-01_18:45:50.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xing Fang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-14 07:08:23	ID requested
1	2020-10-14 07:10:00	announced
⏵ 2	2023-03-01 18:45:51	announced	2023-03-02: Update publication information.

Fang X, Tan T, Gao B, Zhao Y, Liu T, Xia Q, Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells. Front Oncol, 10():537322(2020) [pubmed]

submitter keyword: HBXIP,Germacrone

Quan Xia
contact affiliation	the First Affiliated Hospital of Anhui Medical University
contact email	xiaquan2010@163.com
lab head
Xing Fang
contact affiliation	the First Affiliated Hospital of Anhui Medical University
contact email	1757029857@qq.com
dataset submitter

iProX dataset URI