PXD021960

PXD021960 is an original dataset announced via ProteomeXchange.

Title	IDENTIFICATION OF BIOCHEMICAL AND MOLECULAR MARKERS OF EARLY AGING AND CELLULAR STRESS IN CHILDHOOD CANCER SURVIVORS (CCS) PATIENTS
Description	Survival rates of Childhood Cancer Survivors (CCS) have improved tremendously over the past four decades. Unfortunately, treatments are associated with increased risk for developing early onset of chronic diseases, typical of aging, and secondary cancer. Although several studies have described a phenotype of accelerated aging in CCS, the molecular mechanism underlying the “late effects” remain unknown. Evaluating the mitochondrial metabolism in the mononuclear cells (MNC) isolated from peripheral blood of CCS, age-matched control, and healthy elderly subjects, our data demonstrate that CCS-MNC display an altered aerobic metabolism associated with a low expression of genes involved in mitochondrial biogenesis and regulation. In particular, CCS-MNC show an inefficient oxidative phosphorylation and a low level of NAD+, which determine a low energy production and a metabolic switch to lactate fermentation. The alteration of mitochondrial function is also associated with an increment of lipid peroxidation due to an unbalance among the oxidative stress production and the antioxidantdefenses activation. These biochemical alterations seem linked to the lower expression of CLUH, PGC1-, and SIRT6, which play a pivotal role in the mitochondrial biogenesis and metabolism regulation.Finally, predicting the age by a mathematical model based on the glucose metabolism parameters, CCS display an age similar to that of elderly subjects. The early aging is also confirmed by the increment of aging markers evaluated by a proteomic approach. In conclusion, our data suggest that the early aging described in CCS patients could be due to an alteration of mitochondrial function.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:24:02.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Petretto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-14 01:33:32	ID requested
⏵ 1	2022-02-16 08:24:04	announced

Ravera S, Vigliarolo T, Bruno S, Morandi F, Marimpietri D, Sabatini F, Dagnino M, Petretto A, Bartolucci M, Muraca M, Biasin E, Haupt R, Zecca M, Fagioli F, Cilloni D, Podest, à M, Frassoni F, Identification of Biochemical and Molecular Markers of Early Aging in Childhood Cancer Survivors. Cancers (Basel), 13(20):(2021) [pubmed]

submitter keyword: aerobic metabolism

early aging

mitochondria biogenesis

metabolism modulation

Andrea Petretto
contact affiliation	IRCCS Ist. G. Gaslini
contact email	andreapetretto@gaslini.org
lab head
Andrea Petretto
contact affiliation	Gaslini Children's Hospital
contact email	a.petretto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021960

PRIDE project URI

• PRIDE
  1. PXD021960
     1. Label: PRIDE project
     2. Name: IDENTIFICATION OF BIOCHEMICAL AND MOLECULAR MARKERS OF EARLY AGING AND CELLULAR STRESS IN CHILDHOOD CANCER SURVIVORS (CCS) PATIENTS