PXD021942

PXD021942 is an original dataset announced via ProteomeXchange.

Title	NSMF promotes the replication stress-induced DNA damage response for genome maintenance
Description	Proper activation of DNA repair pathways in response to DNA replication stress is critical for maintaining genomic integrity. Due to the complex nature of the replication fork (RF), problems at the RF require multiple proteins, which remain unidentified, for resolution. In this study, we identified the NMDA receptor synaptonuclear signaling and neuronal migration factor (NSMF) as a key replication stress response factor that is important for ataxia telangiectasia and Rad3-related protein (ATR) activation. NSMF localizes rapidly to stalled RFs and acts as a scaffold to modulate replication protein A (RPA) complex formation with cell division cycle 5-like (CDC5L) and ATR/ATR-interacting protein (ATRIP). Depletion of NSMF compromised phosphorylation and ubiquitination of RPA2 and the ATR signaling cascade, resulting in genomic instability at RFs under DNA replication stress. Consistently, NSMF knockout (KO) mice exhibited increased genomic instability and hypersensitivity to genotoxic stress. NSMF deficiency in human and mouse cells also caused increased chromosomal instability. Collectively, these findings demonstrate that NSMF regulates the ATR pathway and the replication stress response network for genome maintenance and cell survival.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:20:15.270.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Byung-gyu kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-13 02:00:16	ID requested
⏵ 1	2022-02-16 08:20:17	announced

Ju MK, Shin KJ, Lee JR, Khim KW, A Lee E, Ra JS, Kim BG, Jo HS, Yoon JH, Kim TM, Myung K, Choi JH, Kim H, Chae YC, NSMF promotes the replication stress-induced DNA damage response for genome maintenance. Nucleic Acids Res, 49(10):5605-5622(2021) [pubmed]

submitter keyword: replication fork, RPA2, ATR, CDC5L, LC-MS/MS

Hongtae Kim
contact affiliation	School of Life Sciences, Ulsan National University of Science and Technology (UNIST), Ulsan 44919, Republic of Korea
contact email	khtcat@unist.ac.kr
lab head
Byung-gyu kim
contact affiliation	Center for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact email	goldenlion@ibs.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021942

PRIDE project URI

• PRIDE
  1. PXD021942
     1. Label: PRIDE project
     2. Name: NSMF promotes the replication stress-induced DNA damage response for genome maintenance