PXD021938

PXD021938 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry of circulating antigens products isolated from sera of mice infected with Toxoplasma gondii tachyzoites
Description	Toxoplasma gondii is a ubiquitous apicomplexan protozoan parasite that can infect all warm-blooded animals and lead to toxoplasmosis. Discovering efficient methods for diagnosing T. gondii infection and developing vaccine candidates are essential for controlling this disease. Circulating antigens (CAg) are reliable diagnostic indicators of acute infection and highly immunogenic compounds that induce protective immunity. We aimed to identify the CAg spectrum in infected mouse serum. Among 31 identified CAg, we evaluated the ability of RuvB-like helicase (TgRuvBL1), ribonuclease (TgRNaseH1), and ribosomal protein RPS2 (TgRPS2) to protect against T. gondii infection. These three proteins were found in the serum of infected mice. Vaccination with rTgRuvBL1 or rTgRPS2 elicited powerful humoral and cellular immune responses in mice, as levels of total IgG, IgG2a, IgG1, and CD4+ and CD8+ T lymphocytes were significantly increased. The production of proinflammatory gamma-interferon, interleukin-2, and interleukin-12 was also significantly increased. The survival of mice immunized with rTgRuvBL1 (10.0 ± 0.30 days) or rTgRPS2 (11.5 ± 0.34 days) was significantly prolonged after challenge with virulent T. gondii RH strain (P < 0.05 vs. control). The three proteins are potential diagnostic candidates for acute T. gondii and TgRuvBL1 and TgRPS2 might serve as candidate vaccine antigens against toxoplasmosis.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_06:13:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quan Wang
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-13 01:59:58	ID requested
⏵ 1	2021-01-04 06:13:08	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, acute infection, circulating antigens, proteomics, diagnostic candidates, vaccine

Quan Wang
contact affiliation	Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science
contact email	wangquan@shvri.ac.cn
lab head
Quan Wang
contact affiliation	Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science
contact email	wangquan@shvri.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021938
     1. Label: PRIDE project
     2. Name: Mass spectrometry of circulating antigens products isolated from sera of mice infected with Toxoplasma gondii tachyzoites