PXD021927

PXD021927 is an original dataset announced via ProteomeXchange.

Title	Microbore High Throughput Ion Mobility Enabled Mass Spectrometry for Utilisation of Large Cohort Studies
Description	The deployment of proteomic analysis in clinical studies represents a significant opportunity to detect and validate biomarkers in translational medicine, improve disease understanding and provide baseline information on population health. However, comprehensive proteome studies usually employ nano-scale chromatography and often require several hours of analysis/sample. Here we describe a high throughput LC/MS/MS methodology using 1mm scale chromatography requiring only 15 min/sample, coupled to ion mobility-enabled mass spectrometry. The short run time effected a 6 – fold increase in productivity compared with nano-scale LC/MS. The method demonstrated excellent reproducibility with retention time CVs of less than 0.05 % and peak area reproducibility ranging from 5 – 15%. The 1mm system produced similar chromatographic peak capacity values as the miniaturized system, detecting 90% of the E.coli proteins identified by the 75µm LC/MS system. Application to the analysis of serum samples from a human prostate cancer study resulted in the identification of a total of 533 proteins revealing the differential expression of proteins linked to patients receiving hormone-radiotherapy or surgery.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:37:00.370.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021927
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lee Gethings
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-12 06:55:29	ID requested
1	2022-05-26 05:43:10	announced
2	2023-11-14 08:51:17	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:37:00	announced	2024-10-22: Updated project metadata.

10.6019/PXD021927;

10.1021/ACS.JPROTEOME.0C00821;

submitter keyword: Microbore High-throughput Ion Mobility

Lee Gethings
contact affiliation	Waters, United Kingdom
contact email	lee_gethings@waters.com
lab head
Lee Gethings
contact affiliation	Waters Corporation
contact email	lee_gethings@waters.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD021927

PRIDE project URI

• PRIDE
  1. PXD021927
     1. Label: PRIDE project
     2. Name: Microbore High Throughput Ion Mobility Enabled Mass Spectrometry for Utilisation of Large Cohort Studies