PXD021925

PXD021925 is an original dataset announced via ProteomeXchange.

Title	LPS Tolerance inhibits cellular respiration and induces global changes in the secretome
Description	Inflammatory response plays an essential role in the resolution of infections. However, inflammation can be detrimental to the organism and cause irreparable damage, for example during sepsis, when a “cytokine storm” can lead to multiple organ failure and often ends in death. One of the strongest triggers of inflammatory response is bacterial lipopolysaccharide (LPS), acting mostly through Toll-like receptor 4 (TLR4). Prior or prolonged exposure to LPS, however, can induce the state of “endotoxin tolerance” where the macrophages and monocytes do not respond to new endotoxin challenge. The cellular mechanisms regulating this phenomenon remain elusive. Our comprehensive secreted protein analysis comparing LPS-tolerant and -responsive monocyte/macrophage-like cells combined with the extracellular flux analysis reveals the robust switch from the inflammatory to metabolic protein expression as well as points to the involvement of specific proteins that may regulate the change from the responsive to the tolerant state
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:04:25.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Gillen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-12 06:55:19	ID requested
⏵ 1	2021-09-09 10:04:26	announced

Gillen J, Ondee T, Gurusamy D, Issara-Amphorn J, Manes NP, Yoon SH, Leelahavanichkul A, Nita-Lazar A, LPS Tolerance Inhibits Cellular Respiration and Induces Global Changes in the Macrophage Secretome. Biomolecules, 11(2):(2021) [pubmed]

submitter keyword: LPS Tolerance, RAW 264.7, Macrophages, Secretome

Aleksandra Nita-Lazar
contact affiliation	Senior Investigator Chief, Functional Cellular Networks Section Laboratory of Immune System Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda, MD, USA
contact email	nitalazarau@niaid.nih.gov
lab head
Joseph Gillen
contact affiliation	NIH-NIAID
contact email	joseph.gillen@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021925
     1. Label: PRIDE project
     2. Name: LPS Tolerance inhibits cellular respiration and induces global changes in the secretome