PXD021923

PXD021923 is an original dataset announced via ProteomeXchange.

Title	Identification of proximal SUMO-dependent interactors using SUMO-ID
Description	To study the function of SUMOylation and SUMO-SIM interactions for particular substrates poses several challenges. SUMOylation occurs transiently and often in a small percentage of the total copy numbers of a given substrate. Modified proteins can be readily deSUMOylated and SUMO can be recycled and passed to other substrates. SUMO-SIM interactions are also difficult to analyze due to their weak affinity (Kd 1-100 µM). To overcome these technical issues, we developed SUMO-ID, a new strategy based on Split-TurboID to identify SUMO- interactors of specific substrates dependent on SUMO conjugation or interaction. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors. , the high sensitivity of SUMO-ID allowed to identify novel interactors of SUMOylated SALL1, a less characterized SUMO substrate.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:02:57.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FredrikTrulsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-12 06:55:11	ID requested
1	2021-09-21 06:23:42	announced
⏵ 2	2023-04-11 06:03:00	announced	2023-04-11: Updated project metadata.

Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, Cortazar AR, P, é, rez C, Azkargorta M, Iloro I, Carracedo A, Aransay AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD, Identification of proximal SUMO-dependent interactors using SUMO-ID. Nat Commun, 12(1):6671(2021) [pubmed]

submitter keyword: Human, Turbo-ID, Proximity labeling, SALL1, SUMO-ID, SUMO

AlfredVertegaal
contact affiliation	Cell and Chemical Biology at the Leiden University Medical Center
contact email	Vertegaal@lumc.nl
lab head
FredrikTrulsson
contact affiliation	LUMC
contact email	c.f.b.trulsson@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021923

PRIDE project URI

• PRIDE
  1. PXD021923
     1. Label: PRIDE project
     2. Name: Identification of proximal SUMO-dependent interactors using SUMO-ID