PXD021916
PXD021916 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | System-wide identification and prioritization of enzyme substrates by thermal analysis (SIESTA) |
Description | Despite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying and prioritizing substrate proteins which are modulated in the structural and functional levels through modification. Here we describe a high-throughput unbiased proteomic method called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that enzymatic post-translational modification of substrate proteins might change their thermal stability. SIESTA successfully identifies several known and novel substrate candidates for selenoprotein thioredoxin reductase 1, protein kinase B (AKT1) and poly-(ADP-ribose) polymerase-10 systems in up to a depth of 7,179 proteins. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, open new opportunities in investigating the effect of PTMs on signal transduction and facilitate drug discovery. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-11 |
AnnouncementXML | Submission_2021-04-06_02:11:47.911.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amir Ata Saei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-10-12 04:46:38 | ID requested | |
1 | 2021-01-10 23:29:19 | announced | |
⏵ 2 | 2021-04-06 02:11:48 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33637753. |
Publication List
Saei AA, Beusch CM, Sabatier P, Wells JA, Gharibi H, Meng Z, Chernobrovkin A, Rodin S, N, ä, reoja K, Thorsell AG, Karlberg T, Cheng Q, Lundstr, ö, m SL, Gaetani M, V, é, gv, á, ri Á, Arn, é, r ESJ, Sch, ü, ler H, Zubarev RA, System-wide identification and prioritization of enzyme substrates by thermal analysis. Nat Commun, 12(1):1296(2021) [pubmed] |
Keyword List
submitter keyword: SIESTA, HELA, phpsphoproteomics, thermal profiling, enzyme, AKT1 |
Contact List
Prof. Roman A. Zubarev | |
---|---|
contact affiliation | MBB, Karolinska Institutet |
contact email | Roman.Zubarev@ki.se |
lab head | |
Amir Ata Saei | |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
contact email | amirata.saei.dibavar@ki.se |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD021916 |
PRIDE project URI |
Repository Record List
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