PXD021914

PXD021914 is an original dataset announced via ProteomeXchange.

Title	EBV Latent membrane protein1 modifies EV content
Description	Extracellular vesicles (EV) mediate intercellular communication events and alterations in normal vesicle content contribute to function and disease initiation or progression. The ability to package a variety of cargo and transmit molecular information between cells renders EVs important mediators of cell to cell crosstalk. Latent membrane protein 1 (LMP1) is a chief viral oncoprotein expressed in most Epstein-Barr virus (EBV)-associated cancers and is released from cells at high levels in EVs. LMP1 containing EVs have been demonstrated to promote cell growth, migration, differentiation, and regulate immune cell function. Despite these significant changes in recipient cells induced by LMP1 modified EVs, the mechanism how this viral oncogene modulates the recipient cells towards these phenotypes is not well understood. We hypothesize that LMP1 alters EV content and following uptake of the LMP1-modified EVs by the recipient cells results in the activation of cell signaling pathways and increased gene expression which modulates the biological properties of recipient cell towards a new phenotype. Our results show that LMP1 expression alters the EV protein and microRNA content packaged into EVs.
HostingRepository	PRIDE
AnnounceDate	2020-11-03
AnnouncementXML	Submission_2020-11-02_23:05:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021914
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dingani Nkosi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-12 03:40:26	ID requested
⏵ 1	2020-11-02 23:05:03	announced

Dataset with its publication pending

submitter keyword: EBV, LMP1, exosomes, EVs, extracellular vesicles, HK1

David Meckes
contact affiliation	Department of Biomedical sciences FSU College of Medicine
contact email	david.meckes@med.fsu.edu
lab head
Dingani Nkosi
contact affiliation	FSU
contact email	dingani.nkosi@med.fsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021914
     1. Label: PRIDE project
     2. Name: EBV Latent membrane protein1 modifies EV content