PXD021884 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative Proteomic Analysis of an Alexander Disease Murine Model with Severe Reactive Gliosis |
Description | We performed label-free micro-data-independent acquisition (µDIA) to assay brain proteins from a severe model of Alexander disease (AxD, lethal at postnatal day ~35). Of the 5,005 proteins quantified, we observed upregulation of adipocytokine signaling, PPAR, insulin resistance, and glutathione pathways with concomitant downregulation of platelet activation, ether lipid synthesis, and steroid biosynthesis pathways. To validate these DIA results, we setup a targeted parallel reaction monitoring-mass spectrometry assay (PRM-MS) for selected proteins with altered relative concentrations. The PRM-MS assays confirmed the upward or downward relative concentration differences in 16/16 (100%) of the proteins targeted, with 15/16 (94%) of these proteins reaching statistical significance (P-value < 0.05). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:36:32.434.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Heaven |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | (R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-08 00:07:26 | ID requested | |
⏵ 1 | 2024-10-07 13:36:33 | announced | |
Publication List
10.1016/j.mcpro.2021.100180; |
Heaven MR, Herren AW, Flint DL, Pacheco NL, Li J, Tang A, Khan F, Goldman JE, Phinney BS, Olsen ML, Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis. Mol Cell Proteomics, 21(1):100180(2022) [pubmed] |
Keyword List
submitter keyword: reactive gliosis,Alexander disease, parallel reaction monitoring, data-independent acquisition, brain proteome |
Contact List
Michelle L Olsen |
contact affiliation | Virginia Tech School of Neuroscience Life Sciences Building (LS 1) Room 213, Lab 212 Blacksburg VA, 24061 |
contact email | molsen1@vt.edu |
lab head | |
Michael Heaven |
contact affiliation | CSO |
contact email | mheaven@vulcanbiosciences.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021884
- Label: PRIDE project
- Name: Quantitative Proteomic Analysis of an Alexander Disease Murine Model with Severe Reactive Gliosis