PXD021881

PXD021881 is an original dataset announced via ProteomeXchange.

Title	A novel paracrine induction of RNA Granules by Feline Calicivirus
Description	We are here presenting a new paracrine induction of RNA granules by viruses. Infection by viruses imposes major stress on the host cell. In response to this stress, infected cells can induce several defence mechanisms, which include the activation of stress response pathways and the innate immune response. These often result in an inhibition of translation culminating in the assembly of cytoplasmic granules called stress granules (SGs). SGs assembly follows from liquid phase separation of aggregation-prone proteins such G3BP1 and TIA-1, leading to the sequestration of mRNAs. Because this threatens viral gene expression, viruses need to evade these stress response pathways to propagate. Using feline calicivirus (FCV), surrogate for norovirus, the main virus responsible for gastroenteritis outbreaks worldwide, we previously showed that FCV impairs SGs assembly by cleaving the scaffold protein G3BP1. Interestingly, we observed that uninfected bystander cells assembled G3BP1 granules, suggesting a paracrine response trigged by the infection. We now present evidence that virus-free supernatant generated from infected cells can induce the formation of RNA granules. We have characterised the dynamic of the granules assembly via confocal microscopy. Moreover, we provide an understanding of paracrine granules function and specificity through their affinity purification followed by proteomics and RNAseq analysis of their proteins and mRNAs content. This helps to define rules of assembly and novel functions for paracrine granules highlighting fundamental differences with canonical stress granules.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_08:42:09.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentina Iadevaia
SpeciesList	scientific name: Felis catus; NCBI TaxID: 9685;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-07 07:55:36	ID requested
⏵ 1	2022-03-14 08:42:10	announced

Iadevaia V, Burke JM, Eke L, Moller-Levet C, Parker R, Locker N, Novel stress granule-like structures are induced via a paracrine mechanism during viral infection. J Cell Sci, 135(4):(2022) [pubmed]

submitter keyword: G3BP1, Stress granules, FCV, paracrine

Nicolas Locker
contact affiliation	School of Biosciences and Medicine Faculty of Health and Medical Sciences University of Surrey UK
contact email	n.locker@surrey.ac.uk
lab head
Valentina Iadevaia
contact affiliation	University of Surrey
contact email	v.iadevaia@surrey.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021881
     1. Label: PRIDE project
     2. Name: A novel paracrine induction of RNA Granules by Feline Calicivirus