PXD021874

PXD021874 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15
Description	An in-depth glycoproteomic analysis of Haloferax volcanii has been performed, comparing the wildtype H53 with knockout mutants of aglB and agl15. While AglB-dependent glycosylation has been described to occur under standard culture conditions, the Agl15-dependent glycosylation pathway has previously been described to be only active under low-salt conditions. In this dataset, the largest archaeal glycoproteome could be identified with more than 40 N-glycoproteins and more than 100 N-glycopptides. It reveals not only that both glycosylation pathways can be active under the same conditions but also that the same glycosites can be modified by different glycans from both glycosylation pathways.
HostingRepository	PRIDE
AnnounceDate	2021-06-20
AnnouncementXML	Submission_2021-06-20_14:13:45.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii; NCBI TaxID: 2246;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-07 01:18:37	ID requested
⏵ 1	2021-06-20 14:13:45	announced

Schulze S, Pfeiffer F, Garcia BA, Pohlschroder M, Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea. PLoS Biol, 19(6):e3001277(2021) [pubmed]

submitter keyword: glycosylation, archaea, Haloferax volcanii, glycoproteomics

Mechthild Pohlschroder
contact affiliation	University of Pennsylvania Department of Biology
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021874
     1. Label: PRIDE project
     2. Name: Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15