PXD021864

PXD021864 is an original dataset announced via ProteomeXchange.

Title	AP-MS analysis of human FUBIeS30/FAU WT and non-cleavable G73,4A and G74V mutants
Description	To discover candidate FUBIeS30 proteases, we performed affinity purification from human cell lysates expressing tandem Strep II-hemagglutinin (StHA)-tagged WT or mutant (G73,74A or G74V) FUBIeS30-StHA constructs. Subsequent analysis of the co-purified proteins by mass spectrometry (LC-MS/MS) identified differentially interacting factors. We found that two deubiquitinases, USP10 and USP36, were enriched on the non-cleavable mutants compared to WT and subsequently confirmed that USP36 is involved in FUBIeS30 processing in vivo.
HostingRepository	PRIDE
AnnounceDate	2021-08-05
AnnouncementXML	Submission_2021-08-05_02:28:38.081.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludovic Gillet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-06 23:00:54	ID requested
⏵ 1	2021-08-05 02:28:38	announced

Dataset with its publication pending

submitter keyword: AP-MS, FUBIeS30, FAU, DDA, DUB

Ulrike Kutay
contact affiliation	Department of Biology, Institute of Biochemistry, ETH Zurich, Switzerland
contact email	ulrike.kutay@bc.biol.ethz.ch
lab head
Ludovic Gillet
contact affiliation	ETH Zurich
contact email	gillet@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD021864

PRIDE project URI

• PRIDE
  1. PXD021864
     1. Label: PRIDE project
     2. Name: AP-MS analysis of human FUBIeS30/FAU WT and non-cleavable G73,4A and G74V mutants