PXD021859

PXD021859 is an original dataset announced via ProteomeXchange.

Title	SIK2-dependent Phosphoproteome and Interactome upon Salmonella infection
Description	HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type or endogenous SIK2-HA-tagged HeLa cells were left uninfected or infected with Salmonella and lysed 1 h post-infection, followed by HA-IP, Tryptic digest, TMT-labeling and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2021-05-05_21:51:09.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Hahn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-06 06:46:19	ID requested
⏵ 1	2021-05-05 21:51:11	announced

Hahn M, Covarrubias-Pinto A, Herhaus L, Satpathy S, Klann K, Boyle KB, M, ü, nch C, Rajalingam K, Randow F, Choudhary C, Dikic I, infection. Proc Natl Acad Sci U S A, 118(19):(2021) [pubmed]

submitter keyword: Homo sapiens (human)

Ivan Dikic
contact affiliation	Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Marcel Hahn
contact affiliation	Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
contact email	marcel.hahn@stud.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021859

PRIDE project URI

• PRIDE
  1. PXD021859
     1. Label: PRIDE project
     2. Name: SIK2-dependent Phosphoproteome and Interactome upon Salmonella infection