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PXD021850

PXD021850 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAtomic structures of respiratory complex III2 complex IV and supercomplex III2-IV from vascular plants
DescriptionMitochondrial complex III (CIII2) and complex IV (CIV) which can associate into a higher-order supercomplex (SC III2 IV) play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII2, CIV and SC III2 IV from Vigna radiata determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII2 and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII2 revealed long-range, coordinated movements across the complex as well as the motion of CIII2s iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H-channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes, generate new mechanistic hypotheses and allow for the generation of more selective agricultural inhibitors.
HostingRepositoryMassIVE
AnnounceDate2021-04-23
AnnouncementXMLSubmission_2021-04-23_11:06:58.921.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Vigna radiata; common name: mung bean; NCBI TaxID: 157791;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-05 15:18:18ID requested
12021-04-23 11:07:00announced
Publication List
Maldonado M, Guo F, Letts JA, -IV from vascular plants. Elife, 10():(2021) [pubmed]
Keyword List
submitter keyword: respiratory complex
Contact List
James A. Letts
contact affiliationUC Davis Department of Molecular and Cellular Biology
contact emailjaletts@ucdavis.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
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