PXD021850
PXD021850 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Atomic structures of respiratory complex III2 complex IV and supercomplex III2-IV from vascular plants |
| Description | Mitochondrial complex III (CIII2) and complex IV (CIV) which can associate into a higher-order supercomplex (SC III2 IV) play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII2, CIV and SC III2 IV from Vigna radiata determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII2 and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII2 revealed long-range, coordinated movements across the complex as well as the motion of CIII2s iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H-channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes, generate new mechanistic hypotheses and allow for the generation of more selective agricultural inhibitors. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-04-23 |
| AnnouncementXML | Submission_2021-04-23_11:06:58.921.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Vigna radiata; common name: mung bean; NCBI TaxID: 157791; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-10-05 15:18:18 | ID requested | |
| ⏵ 1 | 2021-04-23 11:07:00 | announced |
Publication List
| Maldonado M, Guo F, Letts JA, -IV from vascular plants. Elife, 10():(2021) [pubmed] |
Keyword List
| submitter keyword: respiratory complex |
Contact List
| James A. Letts | |
|---|---|
| contact affiliation | UC Davis Department of Molecular and Cellular Biology |
| contact email | jaletts@ucdavis.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086237/ |
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