PXD021825

PXD021825 is an original dataset announced via ProteomeXchange.

Title	Site-specific N-linked glycosylation of recombinant SARS-CoV-1 and -2 Receptor Binding Domain from GnTI-/- cells
Description	Fusion proteins of the SARS-CoV-1 and SARS-CoV-2 spike receptor binding domain with a fluorescent protein were created in monomeric and trimeric form as tools for receptor binding studies in cultured cells and animal tissues. Here, site specific N-linked glycosylation in the proteins expressed from GnTI-/- cells is profiled with LC-MS/MS, using electron transfer high-energy collision dissociation
HostingRepository	PRIDE
AnnounceDate	2021-02-02
AnnouncementXML	Submission_2021-02-01_23:12:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	glycosylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:51:16	ID requested
⏵ 1	2021-02-01 23:12:50	announced

Dataset with its publication pending

ProteomeXchange project tag: Covid-19

submitter keyword: COVID19, SARS-CoV-2, RBD, EThcD, N-linked glycosylation

Joost Snijder
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Department of Chemistry, Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD021825

PRIDE project URI

• PRIDE
  1. PXD021825
     1. Label: PRIDE project
     2. Name: Site-specific N-linked glycosylation of recombinant SARS-CoV-1 and -2 Receptor Binding Domain from GnTI-/- cells