PXD021818

PXD021818 is an original dataset announced via ProteomeXchange.

Title	Ubiquitinated proteome of E15.5 murine cortical neurons
Description	TRIM9 and TRIM67 are neuronally-enriched E3 ubiquitin ligases essential for neuronal morphogenesis and responses to the axon guidance cue netrin-1. Deletion of either gene in the mouse results in subtle neuroanatomical anomalies yet overt deficits in spatial learning and memory. The identify of few TRIM9 or TRIM67 substrates are known. Here we performed ubiquitin remnant profiling approach (Xu et al., 2010)in cultured cortical neurons from murine wildtype, Trim9-/-, Trim67-/-, and Trim9-/-:Trim67-/- embryos cultured with or without netrin-1 supplementation to attempt to identify proteins with altered ubiquitination. Although batch variability hindered our ability to identify differential protein ubiquitination, the results delineate the neuronal ubiquitionome during neurite outgrowth, axon specification, and axon branching.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_07:09:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephanie Gupton
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:50:28	ID requested
⏵ 1	2021-01-04 07:09:31	announced

submitter keyword: Embryonic cortical neurons, Orbitrap Fusion Lumos, ubiquitinate proteome

Stephanie L. Gupton
contact affiliation	Cell Biology and Physiology, University of North Carolina at Chapel Hill, North Carolina, USA
contact email	sgupton@email.unc.edu
lab head
Stephanie Gupton
contact affiliation	University of North Carolina, Chapel Hill
contact email	sgupton@email.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021818
     1. Label: PRIDE project
     2. Name: Ubiquitinated proteome of E15.5 murine cortical neurons