PXD021818 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ubiquitinated proteome of E15.5 murine cortical neurons |
Description | TRIM9 and TRIM67 are neuronally-enriched E3 ubiquitin ligases essential for neuronal morphogenesis and responses to the axon guidance cue netrin-1. Deletion of either gene in the mouse results in subtle neuroanatomical anomalies yet overt deficits in spatial learning and memory. The identify of few TRIM9 or TRIM67 substrates are known. Here we performed ubiquitin remnant profiling approach (Xu et al., 2010)in cultured cortical neurons from murine wildtype, Trim9-/-, Trim67-/-, and Trim9-/-:Trim67-/- embryos cultured with or without netrin-1 supplementation to attempt to identify proteins with altered ubiquitination. Although batch variability hindered our ability to identify differential protein ubiquitination, the results delineate the neuronal ubiquitionome during neurite outgrowth, axon specification, and axon branching. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-04 |
AnnouncementXML | Submission_2021-01-04_07:09:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephanie Gupton |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-05 03:50:28 | ID requested | |
⏵ 1 | 2021-01-04 07:09:31 | announced | |
Publication List
Keyword List
submitter keyword: Embryonic cortical neurons, Orbitrap Fusion Lumos, ubiquitinate proteome |
Contact List
Stephanie L. Gupton |
contact affiliation | Cell Biology and Physiology, University of North Carolina at Chapel Hill, North Carolina, USA |
contact email | sgupton@email.unc.edu |
lab head | |
Stephanie Gupton |
contact affiliation | University of North Carolina, Chapel Hill |
contact email | sgupton@email.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021818
- Label: PRIDE project
- Name: Ubiquitinated proteome of E15.5 murine cortical neurons