PXD021815

PXD021815 is an original dataset announced via ProteomeXchange.

Title	Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization
Description	SMAC/DIABLO and HTRA2 are mitochondrial proteins whose N-termina sequences, known as inhibitor of apoptosis binding motifs (IBMs), bind and activate ubiquitin ligases knwon as inhibitor of apoptosis proteins (IAPs), unleashing a cell´s poteintial.IBMs comprise a 4-residue, loose consensus sequence, and binding to IAPs requires an unmodified N-terminus. Closely related, IBM-like N-termini, are present in approximately 5% of human proteins. We show that suppression of the N-alpha-acetytransferase NatA truns these cryptic IBM-like sequences into very efficient IAP binders in cell lysates and in vitro, and ultimately also triggers apoptosis. Thus, N-terminal acetylation of IBM-like motifs in NatA substrates shields them from IAPs. This previously unrecognized relatonship suggests that N-terminal acetylation is generally protective against protein degradation in human cells. It also identifies IAPs as agents of a general quality control mechanism targeting unacetylated rogues in metazoans
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:45:53.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bange
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:50:14	ID requested
⏵ 1	2024-10-07 10:45:54	announced

Dataset with its publication pending

submitter keyword: N-terminal acetylation, BIRC3, BIRC2, NatB,N-terminomics, NatA, apoptosis, XIAP, N-degron

Andrea Musacchio
contact affiliation	MPI of Molecular Physiology
contact email	andrea.musacchio@mpi-dortmund.mpg.de
lab head
Tanja Bange
contact affiliation	LMU Munich
contact email	tanja.bange@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021815

PRIDE project URI

• PRIDE
  1. PXD021815
     1. Label: PRIDE project
     2. Name: Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization