PXD021813

PXD021813 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Melg1, Melg2, Melg3, and Tass1
Description	In most eukaryotes, the meiotic chromosomal bouquet (comprising clustered chromosome ends) provides an ordered chromosome arrangement that facilitates pairing and recombination between homologous chromosomes. In the protist Tetrahymena thermophila, the meiotic prophase nucleus stretches enormously and chromosomes assume a bouquet-like arrangement in which telomeres and centromeres are attached to opposite poles of the nucleus. We have identified and characterized three meiosis-specific genes (MELG1-3) that control nuclear elongation and centromere and telomere clustering. Hence, to find out potential interactions, we did LC-MS/MS analysis for Melg1, Melg2, Melg3, and Tass1 (a partner of Melg3) immunoprecipitation samples.
HostingRepository	PRIDE
AnnounceDate	2020-11-23
AnnouncementXML	Submission_2020-11-23_07:34:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao Tian
SpeciesList	scientific name: Tetrahymena thermophila; NCBI TaxID: 5911;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:50:03	ID requested
⏵ 1	2020-11-23 07:34:06	announced

Tian M, Agreiter C, Loidl J, Spatial constraints on chromosomes are instrumental to meiotic pairing. J Cell Sci, 133(22):(2020) [pubmed]

submitter keyword: Tetrahymena thermophila, Meiosis, Immunoprecipitation, LC-MS/MS

Josef Loidl
contact affiliation	Max Perutz Labs Dept. Chromosome Biology University of Vienna
contact email	josef.loidl@univie.ac.at
lab head
Miao Tian
contact affiliation	Max F. Perutz Laboratories
contact email	tian.miao@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021813

PRIDE project URI

• PRIDE
  1. PXD021813
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Melg1, Melg2, Melg3, and Tass1