PXD021804

PXD021804 is an original dataset announced via ProteomeXchange.

Title	Identification of interaction partners of PRMT6
Description	To identify transcription factors of PRMT6 which could recruit PRMT6 to target genes, we used affinity purification of avi-tagged PRMT6 in combination with stable isotope labeling of amino acids in cell culture (SILAC) based mass spectrometry. For this, K562 cells were transduced with a lentiviral co expression vector for the BirA-ligase and avi-PRMT6 with a 21 amino acid taq, which is biotinylated in the cells. Cells expressing the BirA-ligase only served as a control. Avi-PRMT6 cells were grown in heavy SILAC medium and control cells in light SILAC medium for seven passages. Nuclear extracts were prepared from 1x108 cells and subjected to avi-PRMT6 affinity purification using magnetic streptavidin beads. Subsequently, the proteins were eluted from the beads. The eluates from the avi-PRMT6 and the control were mixed in a one to one ratio. Subsequently, we performed quantitative mass spectrometry (MS)-based analysis of the PRMT6 interactome.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_09:54:56.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jörn Lausen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 00:26:45	ID requested
⏵ 1	2021-09-09 09:54:58	announced

Schneider L, Herkt S, Wang L, Feld C, Wesely J, Kuvardina ON, Meyer A, Oellerich T, H, ä, upl B, Seifried E, Bonig H, Lausen J, PRMT6 activates cyclin D1 expression in conjunction with the transcription factor LEF1. Oncogenesis, 10(5):42(2021) [pubmed]

submitter keyword: Human, Hematopoiesis, K562, LC-MS/MS

Joern Lausen
contact affiliation	Universität Stuttgart Institut für Industrielle Genetik Abteilung Eukaryotengenetik Allmandring 31 70569 Stuttgart Germany
contact email	joern.lausen@iig.uni-stuttgart.de
lab head
Jörn Lausen
contact affiliation	Universität Stuttgart Institut für Industrielle Genetik Abteilung Eukaryotengenetik Allmandring 31 70569 Stuttgart
contact email	joern.lausen@iig.uni-stuttgart.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021804

PRIDE project URI

• PRIDE
  1. PXD021804
     1. Label: PRIDE project
     2. Name: Identification of interaction partners of PRMT6