PXD021787

PXD021787 is an original dataset announced via ProteomeXchange.

Title	Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002
Description	Cyanobacteria possess unique intracellular organization. Many proteomic studies have examined different features of cyanobacteria to learn about the structure-function relationships between the intracellular structures of cyanobacteria and their roles in cells. While these studies have made great progress in understanding cyanobacterial physiology, the previous fractionation methods used to purify cellular structures have limitations; specifically, certain regions of cells cannot be purified with existing fractionation methods. Proximity-based proteomics techniques were developed to overcome the limitations of biochemical fractionation for proteomics. Proximity-based proteomics relies on spatiotemporal protein labeling followed by mass spectrometry of the labeled proteins to determine the proteome of the region of interest. We have performed proximity-based proteomics in the cyanobacterium Synechococcus sp. PCC 7002 with the APEX2 enzyme, an engineered ascorbate peroxidase. We determined the proteome of the thylakoid lumen, a region of the cell that has remained challenging to study with existing methods, using a translational fusion between APEX2 and PsbU, a lumenal subunit of photosystem II. Our results demonstrate the power of APEX2 as a tool to study the cell biology of intracellular features and processes, including PSII assembly in cyanobacteria with enhanced spatiotemporal resolution.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_07:04:31.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kelsey Dahlgren
SpeciesList	scientific name: Synechococcus sp. PCC 7002; NCBI TaxID: 32049;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-02 00:57:44	ID requested
⏵ 1	2021-09-09 07:04:31	announced

Dahlgren KK, Gates C, Lee T, Cameron JC, Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002. Photosynth Res, 147(2):177-195(2021) [pubmed]

submitter keyword: Proximity-based Proteomics, APEX2, cyanobacteria, thylakoid lumen, photosynthesis

Jeffrey Carlyle Cameron
contact affiliation	Department of Biochemistry, University of Colorado, Boulder, CO 80309, USA Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO 80309, USA National Renewable Energy Laboratory, Golden, CO 80401, USA
contact email	Jeffrey.C.Cameron@colorado.edu
lab head
Kelsey Dahlgren
contact affiliation	Biochemistry Department at University of Colorado-Boulder
contact email	kelsey.dahlgren@colorado.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021787
     1. Label: PRIDE project
     2. Name: Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002