PXD021782

PXD021782 is an original dataset announced via ProteomeXchange.

Title	Identification of N-linked glycosylated peptides in the open ocean diatom Thalassiosira oceanica
Description	N-linked glycosylation is a posttranslational modification affecting protein folding and protein function. The N-linked glycosylation pathway in algae is poorly characterized, and further knowledge is needed to understand the cell biology of algae and the evolution of N-linked glycosylation. This study investigated the N-linked glycosylation pathway in the open ocean diatom Thalassiosira oceanica and identified N-linked glycosylated peptides through solid-phase extraction of N-linked glycosylated peptides (SPEG). We characterized 118 N-linked glycosylated peptides from samples of cells grown in high- and low-iron conditions. The identified peptides had 81% NXT-type motifs, with X being any amino acids except proline. The presence of N-linked glycosylation sites in the iron starvation-induced protein 1a (ISIP1a) confirmed its predicted topology, contributing to the biochemical characterization of ISIP1 proteins.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_07:30:29.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Cohen
SpeciesList	scientific name: Thalassiosira oceanica; NCBI TaxID: 159749;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-02 00:40:57	ID requested
⏵ 1	2021-09-09 07:30:30	announced

Behnke J, Cohen AM, LaRoche J, N-linked glycosylation enzymes in the diatom Thalassiosira oceanica exhibit a diel cycle in transcript abundance and favor for NXT-type sites. Sci Rep, 11(1):3227(2021) [pubmed]

submitter keyword: N-linked glycosylation, Diatom, Solid-phase extraction of N-linked glycosylated peptides

Julie LaRoche
contact affiliation	Professor. Tier 1 Canada Research Chair in Marine Microbial Genomics and Biogeochemistry. Department of Biology, Dalhousie University
contact email	Julie.Laroche@Dal.Ca
lab head
Alejandro Cohen
contact affiliation	Dalhousie University. Biological Mass Spectrometry Core Facility
contact email	alejandro.cohen@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021782
     1. Label: PRIDE project
     2. Name: Identification of N-linked glycosylated peptides in the open ocean diatom Thalassiosira oceanica