PXD021781

PXD021781 is an original dataset announced via ProteomeXchange.

Title	Profile of growth factors in calf costochondral junction – the site of initial bone formation
Description	Endochondral bone formation is orchestrated by growth factors produced by chondrocytes and deposited in cartilage matrix. Some of these factors were identified, but their complete list and relationship remains unknown. In this work the growth factors were isolated from calcified cartilage of costochondral junctions. The isolation involved hyaluronidase digestion, guanidinium hydrochloride (GuHCl) extraction, HCl decalcification and GuHCl extraction of decalcified matrix. Growth factors were purified by heparin chromatography and estimated by enzyme-linked immunosorbent assay (ELISA) or used for protein sequence analysis. Bone morphogenetic protein-7 (BMP-7), Growth/differentiation factor 5 (GDF-5), and NEL-like protein 1 (NELL-1) quantitatively dominated in material obtained in all steps of isolation. During ossification perivascular cells and septoclasts enter the cartilage and release growth factors stimulating osteoclastogenesis. Osteoclasts dissolve calcified cartilage and transport released factors needed for stimulation of osteoprogenitor cells. Presence of BMP-7, GDF-5 and NELL-1 at the site of initial bone formation may suggest that their synergistic action favors osteogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-02_23:24:08.816.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021781
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Kistowski
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-02 00:32:42	ID requested
⏵ 1	2021-05-02 23:24:09	announced

Iwan A, Moskalewski S, Hyc A, Growth factor profile in calcified cartilage from the metaphysis of a calf costochondral junction, the site of initial bone formation. Biomed Rep, 14(6):54(2021) [pubmed]

submitter keyword: endochondral bone formation, calcified cartilage, growth factors, osteoclasts

Michał Dadlez
contact affiliation	Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics PAS
contact email	michald@ibb.waw.pl
lab head
Michał Kistowski
contact affiliation	Institute of Biochemistry and Biophysics PAS
contact email	mkisto@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021781
     1. Label: PRIDE project
     2. Name: Profile of growth factors in calf costochondral junction – the site of initial bone formation