PXD021774 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Translation in human mature erythrocytes |
Description | It is generally believed that human mature erythrocytes do not possess functional ribosomes, and therefore cannot synthesize proteins. However, this dogma is not consistent with the long life of mature erythrocytes in the circulatory system. The mass-spectrometry analysis was done on highly pure preparation of human mature erythrocytes to identify the proteome. Results of the analysis show that there is no contamination from other cell types. We also demonstrate translation by polysome profiling, metabolic labelling and RiboPuromycylation. RNA-seq and quantitative RT-PCR assays revealed that HBA (alpha globin) and HBB (beta globin) transcripts are selectively translated. RNA-seq and translatome analyses revealed the presence of all necessary translation factors and aminoacyl tRNA synthetases. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:24:32.849.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021774 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ling Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-01 00:25:10 | ID requested | |
1 | 2023-03-10 15:45:18 | announced | |
⏵ 2 | 2023-11-14 08:24:33 | announced | 2023-11-14: Updated project metadata. |
Publication List
Kumar SD, Kar D, Akhtar MN, Willard B, Roy D, Hussain T, Rajyaguru PI, Eswarappa SM, Evidence for low-level translation in human erythrocytes. Mol Biol Cell, 33(12):br21(2022) [pubmed] |
10.6019/PXD021774; |
Keyword List
submitter keyword: Erythrocytes, Globin, Translation, RNA |
Contact List
Sandeep M Eswarappa |
contact affiliation | Indian Institute of Science |
contact email | sandeep@iisc.ac.in |
lab head | |
Ling Li |
contact affiliation | Cleveland Clinic |
contact email | lil5@ccf.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021774
- Label: PRIDE project
- Name: Translation in human mature erythrocytes