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PXD021772

PXD021772 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe trypanosome Variant Surface Glycoprotein mRNA is stabilized by an essential unconventional RNA-binding protein
DescriptionSalivarian trypanosomes cause human sleeping sickness and economically important livestock diseases, transmitted by Tsetse flies and replicating extracellularly throughout the life cycle. The surface of "bloodstream forms", which live within mammals, is coated by a monolayer of a Variant Surface Glycoprotein (VSG). Switching of the expressed VSG gene enables the parasites to evade adaptive immunity. Up until now, the mechanism by which VSG mRNA stability is maintained was unknown. To identify proteins specifically interacting with VSG mRNA (and alpha tubulin as control), we performed RNA-antisense purifications (RAP) following instructions published by McHugh et al. 2018 with some modifications.
HostingRepositoryPRIDE
AnnounceDate2021-04-14
AnnouncementXMLSubmission_2021-04-14_09:03:36.461.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Ruppert
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-01 00:11:57ID requested
12021-04-14 09:03:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: trypanosomes, bloodstream forms,VSG,
Contact List
Thomas Ruppert
contact affiliationZentrum für Molekulare Biologie (ZMBH), Mass Spectrometry and Proteomics
contact emailt.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliationZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact emailt.ruppert@zmbh.uni-heidelberg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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