PXD021772

PXD021772 is an original dataset announced via ProteomeXchange.

Title	The trypanosome Variant Surface Glycoprotein mRNA is stabilized by an essential unconventional RNA-binding protein
Description	Salivarian trypanosomes cause human sleeping sickness and economically important livestock diseases, transmitted by Tsetse flies and replicating extracellularly throughout the life cycle. The surface of "bloodstream forms", which live within mammals, is coated by a monolayer of a Variant Surface Glycoprotein (VSG). Switching of the expressed VSG gene enables the parasites to evade adaptive immunity. Up until now, the mechanism by which VSG mRNA stability is maintained was unknown. To identify proteins specifically interacting with VSG mRNA (and alpha tubulin as control), we performed RNA-antisense purifications (RAP) following instructions published by McHugh et al. 2018 with some modifications.
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-14_09:03:36.461.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-01 00:11:57	ID requested
⏵ 1	2021-04-14 09:03:36	announced

Dataset with its publication pending

submitter keyword: trypanosomes, bloodstream forms,VSG,

Thomas Ruppert
contact affiliation	Zentrum für Molekulare Biologie (ZMBH), Mass Spectrometry and Proteomics
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021772

PRIDE project URI

• PRIDE
  1. PXD021772
     1. Label: PRIDE project
     2. Name: The trypanosome Variant Surface Glycoprotein mRNA is stabilized by an essential unconventional RNA-binding protein