PXD021759 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Catechol-modified protein extracts from human heart mitochondria after APBA chromatography |
Description | Catecholamine metabolism via monoamine oxidase (MAO) contributes to cardiac injury in models of ischemia and diabetes, but the pathogenic mechanisms involved are unclear. MAO deaminates norepinephrine (NE) and dopamine (DA) to produce H2O2 and highly reactive ‘catecholaldehydes,’ which may be toxic to mitochondria due to the localization of MAO to the outer mitochondrial membrane. We performed a comprehensive analysis of catecholamine metabolism and its impact on mitochondrial energetics in atrial myocardium obtained from patients with and without type 2 diabetes. This data set contains a list of human cardiac mitochondrial proteins that were isolated following aminophenylboronic acid (APBA) column chromatography, which allows for selective purification of catechol-modified proteins. Samples of human heart mitochondria were prepared from discarded atrial appendage biopsies taken during elective cardiac surgery. Following extraction via APBA, bands were excised from a gel and subjected to proteomics analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-21 |
AnnouncementXML | Submission_2020-10-20_22:36:28.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021759 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | robert Pope |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-30 01:14:58 | ID requested | |
⏵ 1 | 2020-10-20 22:36:29 | announced | |
Publication List
Nelson MM, Efird JT, Kew KA, Katunga LA, Monroe TB, Doorn JA, Beatty CN, Shi Q, Akhter SA, Alwair H, Robidoux J, Anderson EJ, Enhanced Catecholamine Flux and Impaired Carbonyl Metabolism Disrupt Cardiac Mitochondrial Oxidative Phosphorylation in Diabetes Patients. Antioxid Redox Signal, 35(4):235-251(2021) [pubmed] |
Keyword List
submitter keyword: human, catecholamine metabolism, MAO, atrial mitocardium, mitochondrial proteomics, type 2 diabetes, cardiomyopathy, atrial tissue |
Contact List
Ethan J. Anderson |
contact affiliation | Associate Professor Pharmaceutical Sciences & Experimental Therapeutics College of Pharmacy FOE Diabetes Research Center University of Iowa |
contact email | ethan-anderson@uiowa.edu |
lab head | |
robert Pope |
contact affiliation | University of Iowa, COM, Iowa City, IA 52241 |
contact email | marshall-pope@uiowa.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021759
- Label: PRIDE project
- Name: Catechol-modified protein extracts from human heart mitochondria after APBA chromatography