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PXD021759

PXD021759 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCatechol-modified protein extracts from human heart mitochondria after APBA chromatography
DescriptionCatecholamine metabolism via monoamine oxidase (MAO) contributes to cardiac injury in models of ischemia and diabetes, but the pathogenic mechanisms involved are unclear. MAO deaminates norepinephrine (NE) and dopamine (DA) to produce H2O2 and highly reactive ‘catecholaldehydes,’ which may be toxic to mitochondria due to the localization of MAO to the outer mitochondrial membrane. We performed a comprehensive analysis of catecholamine metabolism and its impact on mitochondrial energetics in atrial myocardium obtained from patients with and without type 2 diabetes. This data set contains a list of human cardiac mitochondrial proteins that were isolated following aminophenylboronic acid (APBA) column chromatography, which allows for selective purification of catechol-modified proteins. Samples of human heart mitochondria were prepared from discarded atrial appendage biopsies taken during elective cardiac surgery. Following extraction via APBA, bands were excised from a gel and subjected to proteomics analysis.
HostingRepositoryPRIDE
AnnounceDate2020-10-21
AnnouncementXMLSubmission_2020-10-20_22:36:28.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021759
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterrobert Pope
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-30 01:14:58ID requested
12020-10-20 22:36:29announced
Publication List
Nelson MM, Efird JT, Kew KA, Katunga LA, Monroe TB, Doorn JA, Beatty CN, Shi Q, Akhter SA, Alwair H, Robidoux J, Anderson EJ, Enhanced Catecholamine Flux and Impaired Carbonyl Metabolism Disrupt Cardiac Mitochondrial Oxidative Phosphorylation in Diabetes Patients. Antioxid Redox Signal, 35(4):235-251(2021) [pubmed]
Keyword List
submitter keyword: human, catecholamine metabolism, MAO, atrial mitocardium, mitochondrial proteomics, type 2 diabetes, cardiomyopathy, atrial tissue
Contact List
Ethan J. Anderson
contact affiliationAssociate Professor Pharmaceutical Sciences & Experimental Therapeutics College of Pharmacy FOE Diabetes Research Center University of Iowa
contact emailethan-anderson@uiowa.edu
lab head
robert Pope
contact affiliationUniversity of Iowa, COM, Iowa City, IA 52241
contact emailmarshall-pope@uiowa.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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