PXD021756

PXD021756 is an original dataset announced via ProteomeXchange.

Title	The Serpin-like Loop-insertion of Ovalbumin Increases Stability and Reduces OVA 323-339 Epitope Immunogenicity
Description	Chicken ovalbumin (cOVA) has been studied for decades primarily due to the robust genetic and molecular resources that are available for experimental investigations. cOVA is a member of the serpin superfamily of proteins that function as protease inhibitors, although cOVA does not exhibit this activity. As a serpin, cOVA possesses a protease-sensitive reactive-center loop that lies adjacent to the OVA 323-339 CD4+ T-cell epitope. We took advantage of the previously described single-substitution-variant, OVA R339T, which can undergo the dramatic structural transition observed in serpins to study how changes in loop size and protein stability influences CD4+ T-cell priming in vivo. We observed that the OVA R339T loop-insertion increases stability and protease resistance, resulting in reduced CD4+ T-cell priming of the OVA 323-339 epitope in SJL mice. These findings have implications for the design of more effective vaccines for the treatment of infectious diseases and cancer as well as the development of more robust CD4+ T-cell-epitope prediction tools.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:38.405.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021756
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniel Moss
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-30 01:01:19	ID requested
1	2021-05-07 09:20:33	announced
⏵ 2	2023-11-14 08:51:38	announced	2023-11-14: Updated project metadata.

10.6019/PXD021756;

submitter keyword: LC-MS, Antigen Processing, Limited Proteolysis

Samuel J. Landy
contact affiliation	Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, Louisiana 70112
contact email	landry@tulane.edu
lab head
Daniel Moss
contact affiliation	Tulane University
contact email	dmoss2@tulane.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021756
     1. Label: PRIDE project
     2. Name: The Serpin-like Loop-insertion of Ovalbumin Increases Stability and Reduces OVA 323-339 Epitope Immunogenicity