PXD021751

PXD021751 is an original dataset announced via ProteomeXchange.

Title	An unbiased approach to mapping the signaling network of the pseudorabies virus US3 protein
Description	The US3 serine/threonine protein kinase is conserved among the alphaherpesvirus family and represents an important virulence factor. US3 plays a role in viral nuclear egress, induces dramatic alterations of the cytoskeleton, represses apoptosis, enhances gene expression and modulates the immune response. Although several substrates of US3 have been identified, an unbiased screen to identify US3 phosphorylation targets has not yet been described. Here we perform a shotgun and phosphoproteomics analysis of cells expressing the US3 protein of pseudorabies virus (PRV) to identify US3 phosphorylation targets in an unbiased way. These results provide new insight in the signaling network of the US3 protein kinase and may serve as a basis for future research into the role of the US3 protein in the viral replication cycle.
HostingRepository	PRIDE
AnnounceDate	2020-11-24
AnnouncementXML	Submission_2020-11-23_22:58:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Delphi Van Haver
SpeciesList	scientific name: Sus scrofa; NCBI TaxID: 9823;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-30 00:13:27	ID requested
⏵ 1	2020-11-23 22:58:02	announced

Jansens RJJ, Marmiroli S, Favoreel HW, An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein. Pathogens, 9(11):(2020) [pubmed]

submitter keyword: Pseudorabies virus, PRV, US3, kinase, alphaherpesvirus, phosphoproteome

Herman Favoreel
contact affiliation	Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium
contact email	herman.favoreel@ugent.be
lab head
Delphi Van Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021751
     1. Label: PRIDE project
     2. Name: An unbiased approach to mapping the signaling network of the pseudorabies virus US3 protein