PXD021743 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide |
Description | We investigated the conformational dynamics of the LPS translocon LptDE was using hydrogen-deuterium exchange mass spectrometry. We evaluated the conformational changes of LptDE upon binding of LPS, Re-LPS (an LPS substructure), thanatin, and the membrane lipid POPG. Our data reveal that LPS induces opening of the LptD β-taco domain, coupled with conformational changes on β-strands adjacent to the putative lateral exit gate. Conversely, an antimicrobial peptide, thanatin, stabilises the β-taco, thus blocking these conformational fluctuations and preventing LPS transport. Our results illustrate that LPS insertion into the OM relies on concerted opening movements of both β-barrel and β-taco domains. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-16 |
AnnouncementXML | Submission_2020-11-23_07:35:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Francesco Fiorentino |
SpeciesList | scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; |
ModificationList | No PTMs are included in the dataset |
Instrument | SYNAPT G2-Si |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-29 06:32:47 | ID requested | |
1 | 2020-11-16 14:50:24 | announced | |
⏵ 2 | 2020-11-23 07:35:49 | announced | 2020-11-23: Updated publication reference for PubMed record(s): 33199913. |
Publication List
Fiorentino F, Sauer JB, Qiu X, Corey RA, Cassidy CK, Mynors-Wallis B, Mehmood S, Bolla JR, Stansfeld PJ, Robinson CV, Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide. Nat Chem Biol, 17(2):187-195(2021) [pubmed] |
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
submitter keyword: HDX-MS, hydrogen-deuterium exchange, LPS, LptDE, thanatin |
Contact List
Carol V. Robinson |
contact affiliation | Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK |
contact email | carol.robinson@chem.ox.ac.uk |
lab head | |
Francesco Fiorentino |
contact affiliation | University of Oxford |
contact email | francesco.fiorentino@chem.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021743
- Label: PRIDE project
- Name: Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide