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PXD021743

PXD021743 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamics of an LPS translocon induced by substrate and an antimicrobial peptide
DescriptionWe investigated the conformational dynamics of the LPS translocon LptDE was using hydrogen-deuterium exchange mass spectrometry. We evaluated the conformational changes of LptDE upon binding of LPS, Re-LPS (an LPS substructure), thanatin, and the membrane lipid POPG. Our data reveal that LPS induces opening of the LptD β-taco domain, coupled with conformational changes on β-strands adjacent to the putative lateral exit gate. Conversely, an antimicrobial peptide, thanatin, stabilises the β-taco, thus blocking these conformational fluctuations and preventing LPS transport. Our results illustrate that LPS insertion into the OM relies on concerted opening movements of both β-barrel and β-taco domains.
HostingRepositoryPRIDE
AnnounceDate2020-11-16
AnnouncementXMLSubmission_2020-11-23_07:35:49.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancesco Fiorentino
SpeciesList scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
ModificationListNo PTMs are included in the dataset
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-29 06:32:47ID requested
12020-11-16 14:50:24announced
22020-11-23 07:35:49announced2020-11-23: Updated publication reference for PubMed record(s): 33199913.
Publication List
Fiorentino F, Sauer JB, Qiu X, Corey RA, Cassidy CK, Mynors-Wallis B, Mehmood S, Bolla JR, Stansfeld PJ, Robinson CV, Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide. Nat Chem Biol, 17(2):187-195(2021) [pubmed]
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)
submitter keyword: HDX-MS, hydrogen-deuterium exchange, LPS, LptDE, thanatin
Contact List
Carol V. Robinson
contact affiliationDepartment of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, UK
contact emailcarol.robinson@chem.ox.ac.uk
lab head
Francesco Fiorentino
contact affiliationUniversity of Oxford
contact emailfrancesco.fiorentino@chem.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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