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PXD021736

PXD021736 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential proteomic analysis in a host–parasite interaction of host cells infected with Toxoplasma gondii
DescriptionToxoplasma gondii, a representative model organism of the phylum Apicomplexa, can infect almost all warm-blooded organisms including humans. Invasion of host cells in a host–parasite interaction is the key step necessary for T. gondii to complete its life cycle. Here, we investigate global proteomic changes based on TMT analysis in both the host cells (human foreskin fibroblasts, HFFs) and T. gondii during intracellular infection. A total of 3477 and 1434 proteins were quantified, of which 378 and 1100 proteins were differentially expressed (p-value<0.05 and ≥1.5-fold change)in Homo sapiens and T. gondii proteins, respectively. In HFF cells, Gene Ontology (GO) and KEGG pathway analyses revealed a large number of differentially expressed proteins (DEPs) enriched in metabolic processes and immune-associated signaling pathways, such as NF-κB, cAMP, and Rap1 signaling pathways. T. gondii DEPs involved in pathway analysis and GO annotations included cellular protein metabolic process, peptide metabolic process, and peptide biosynthetic processes and indicated that cell biosynthesis and metabolism were increased during T. gondii infection. These findings reveal new proteomic differences in the parasite–host interaction during T. gondii infection.
HostingRepositoryPRIDE
AnnounceDate2021-05-10
AnnouncementXMLSubmission_2021-05-09_22:24:42.855.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterhui sun
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-29 05:25:24ID requested
12021-05-09 22:24:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Toxoplasma gondii
host–parasite interaction
TMT
comparative proteomic
Contact List
hui sun
contact affiliationShandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining City, Shandong Province, 272033, China
contact emailsunhui123aq@126.com
lab head
hui sun
contact affiliationShandong First Medical University & Shandong Academy of Medical Sciences Shandong Institute of Parasitic Diseases
contact emailsunhui123aq@126.com
dataset submitter
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Dataset FTP location
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