PXD021736 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential proteomic analysis in a host–parasite interaction of host cells infected with Toxoplasma gondii |
Description | Toxoplasma gondii, a representative model organism of the phylum Apicomplexa, can infect almost all warm-blooded organisms including humans. Invasion of host cells in a host–parasite interaction is the key step necessary for T. gondii to complete its life cycle. Here, we investigate global proteomic changes based on TMT analysis in both the host cells (human foreskin fibroblasts, HFFs) and T. gondii during intracellular infection. A total of 3477 and 1434 proteins were quantified, of which 378 and 1100 proteins were differentially expressed (p-value<0.05 and ≥1.5-fold change)in Homo sapiens and T. gondii proteins, respectively. In HFF cells, Gene Ontology (GO) and KEGG pathway analyses revealed a large number of differentially expressed proteins (DEPs) enriched in metabolic processes and immune-associated signaling pathways, such as NF-κB, cAMP, and Rap1 signaling pathways. T. gondii DEPs involved in pathway analysis and GO annotations included cellular protein metabolic process, peptide metabolic process, and peptide biosynthetic processes and indicated that cell biosynthesis and metabolism were increased during T. gondii infection. These findings reveal new proteomic differences in the parasite–host interaction during T. gondii infection. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-10 |
AnnouncementXML | Submission_2021-05-09_22:24:42.855.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | hui sun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-29 05:25:24 | ID requested | |
⏵ 1 | 2021-05-09 22:24:43 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Toxoplasma gondii |
host–parasite interaction |
TMT |
comparative proteomic |
Contact List
hui sun |
contact affiliation | Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining City, Shandong Province, 272033, China |
contact email | sunhui123aq@126.com |
lab head | |
hui sun |
contact affiliation | Shandong First Medical University & Shandong Academy of Medical Sciences Shandong Institute of Parasitic Diseases |
contact email | sunhui123aq@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021736
- Label: PRIDE project
- Name: Differential proteomic analysis in a host–parasite interaction of host cells infected with Toxoplasma gondii