PXD021736

PXD021736 is an original dataset announced via ProteomeXchange.

Title	Differential proteomic analysis in a host–parasite interaction of host cells infected with Toxoplasma gondii
Description	Toxoplasma gondii, a representative model organism of the phylum Apicomplexa, can infect almost all warm-blooded organisms including humans. Invasion of host cells in a host–parasite interaction is the key step necessary for T. gondii to complete its life cycle. Here, we investigate global proteomic changes based on TMT analysis in both the host cells (human foreskin fibroblasts, HFFs) and T. gondii during intracellular infection. A total of 3477 and 1434 proteins were quantified, of which 378 and 1100 proteins were differentially expressed (p-value<0.05 and ≥1.5-fold change)in Homo sapiens and T. gondii proteins, respectively. In HFF cells, Gene Ontology (GO) and KEGG pathway analyses revealed a large number of differentially expressed proteins (DEPs) enriched in metabolic processes and immune-associated signaling pathways, such as NF-κB, cAMP, and Rap1 signaling pathways. T. gondii DEPs involved in pathway analysis and GO annotations included cellular protein metabolic process, peptide metabolic process, and peptide biosynthetic processes and indicated that cell biosynthesis and metabolism were increased during T. gondii infection. These findings reveal new proteomic differences in the parasite–host interaction during T. gondii infection.
HostingRepository	PRIDE
AnnounceDate	2021-05-10
AnnouncementXML	Submission_2021-05-09_22:24:42.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hui sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-29 05:25:24	ID requested
⏵ 1	2021-05-09 22:24:43	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii

host–parasite interaction

TMT

comparative proteomic

hui sun
contact affiliation	Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining City, Shandong Province, 272033, China
contact email	sunhui123aq@126.com
lab head
hui sun
contact affiliation	Shandong First Medical University & Shandong Academy of Medical Sciences Shandong Institute of Parasitic Diseases
contact email	sunhui123aq@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021736

PRIDE project URI

• PRIDE
  1. PXD021736
     1. Label: PRIDE project
     2. Name: Differential proteomic analysis in a host–parasite interaction of host cells infected with Toxoplasma gondii