PXD021732

PXD021732 is an original dataset announced via ProteomeXchange.

Title	Free rather than total iron content is critically linked to the Fur physiology in Shewanella oneidensis
Description	Ferric uptake regulator (Fur) is a transcriptional regulator playing a central role in iron homeostasis of many bacteria and Fur inactivation commonly results in pleiotropic phenotypes. In Shewanella oneidensis, a representative of dissimilatory metal-reducing -proteobacteria capable of respiring a variety of chemicals as electron acceptors (EAs), the Fur loss substantially impairs respiration. However, to date the mechanism underlying the physiological phenomenon remains obscure. This investigation reveals that Fur loss compromises activity of iron proteins requiring biosynthetic processes for their iron cofactors, heme in particular. We then show that S. oneidensis Fur is critical for maintaining heme homeostasis by affecting both its biosynthesis and decomposition of the molecule. Intriguingly, the abundance of iron-containing proteins controlled by H2O2-responding regulator OxyR increases in the fur mutant because the Fur loss activates OxyR. By comparing suppression of membrane-impermeable, membrane-permeable, and intracellular-only iron chelators on heme deficiency and elevated H2O2 resistance, our data suggest that the elevation of the free iron content by the Fur loss is likely to be the predominant factor for the Fur physiology. Overall, these results provide circumstantial evidence that Fur inactivation disturbs bacterial iron homeostasis by altering transcription of its regulon members, through which many physiological processes, such as respiration and oxidative stress response, are transformed.
HostingRepository	iProX
AnnounceDate	2020-09-29
AnnouncementXML	Submission_2022-09-01_22:20:48.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xue Feng
SpeciesList	scientific name: Shewanella oneidensis MR-1; NCBI TaxID: 211586;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-29 00:29:35	ID requested
⏵ 1	2022-09-01 22:20:49	announced

Liu L, Feng X, Wang W, Chen Y, Chen Z, Gao H, . Front Microbiol, 11():593246(2020) [pubmed]

submitter keyword: iron homeostasis, Shewanella, Fur regulation, Heme,oxidative stress 2

Haichun Gao
contact affiliation	Institute of Microbiology & College of Life Sciences Zhejiang University Hangzhou, China
contact email	haichung@zju.edu.cn
lab head
Xue Feng
contact affiliation	Institute of Microbiology & College of Life Sciences Zhejiang University. Hangzhou, China
contact email	a18171314@zju.edu.cn
dataset submitter

iProX dataset URI