PXD021731

PXD021731 is an original dataset announced via ProteomeXchange.

Title	Proteomics link translational reprogramming to S-glutathionalytion and induced priming during cold acclimation in barley roots
Description	Plants due to their sessile nature rely on root systems to mediate many challenging stimuli. A major cornerstone of the responses are root proteomes, shaped to overcome biotic and abiotic cues. Proteome-wide reprogramming events are magnified in root meristems due to their active protein production. Using root meristems as a test system, we here study the major rewiring that plants face during cold acclimation. To achieve that, we performed tandem mass tag (TMT) label-mediated bottom-up proteomics of two consecutive segments of barley root apexes, comparing changes in global protein contents and individual protein abundances. We report an intricate translational reprogramming happening in the distal apex zone. The reprogramming includes all the translational machinery from ribosome biogenesis to protein folding, even suggesting roles for cold-specific ribosomal protein (RP) paralogs. Finally, this event is accompanied by upregulation of glutathione production and S-glutathionylation assemblage machineries. To summarize, we couple previous reports of an alternatively cold-spliced transcriptome to potential cold-specialized ribosomes and subsequent posttranslational modification (PTM), S-glutathione, of proteins. We propose that these PTMs protect the proteome during cold by acting as ROS scavengers, and consequently, provide a mechanistic link to the cold priming capabilities for root-specific biotic stress.
HostingRepository	PRIDE
AnnounceDate	2021-04-14
AnnouncementXML	Submission_2021-04-14_04:36:24.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuai Nie
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-28 23:37:08	ID requested
⏵ 1	2021-04-14 04:36:25	announced

Dataset with its publication pending

submitter keyword: Barley

cv Keel

proteins

cold acclimation

fungal stress

root meristem

proteomics

ribosome

priming elicitors

chitin

chitosan

Berin A. Boughton
contact affiliation	School of BioSciences, The University of Melbourne Parkville VIC 3010, Australia Australian National Phenome Centre, Murdoch University, Murdoch, WA 6150, Australia
contact email	Berin.Boughton@murdoch.edu.au
lab head
Shuai Nie
contact affiliation	The University of Melbourne
contact email	chemyumen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD021731

PRIDE project URI

• PRIDE
  1. PXD021731
     1. Label: PRIDE project
     2. Name: Proteomics link translational reprogramming to S-glutathionalytion and induced priming during cold acclimation in barley roots