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PXD021728 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCaspase-3 cleaves extracellular vesicle proteins during auditory brainstem development
DescriptionSound localization requires extremely precise development of auditory brainstem circuits, the molecular mechanisms of which are largely unknown. We previously demonstrated a novel requirement for non-apoptotic activity of the protease caspase-3 in chick auditory brainstem development. Here, we used mass spectrometry to identify proteolytic substrates of caspase-3 during chick auditory brainstem development. Functional annotation analysis revealed that our caspase-3 substrates were enriched more than two-fold for proteins associated with extracellular vesicles (EVs), membrane-bound nanoparticles that function in intercellular communication. The proteome of EVs isolated from the auditory brainstem contained caspase-3 and was highly enriched for the caspase-3 substrates identified here. Additionally, we identified two caspase-3 substrates with known functions in axon guidance, namely Neural Cell Adhesion Molecule (NCAM) and Neuronal-glial Cell Adhesion Molecule (Ng-CAM), that were found in auditory brainstem EVs and expressed in the auditory pathway alongside cleaved caspase-3. Taken together, these data suggest a novel developmental mechanism whereby caspase-3 influences auditory brainstem circuit formation through the proteolytic cleavage of EV proteins.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterForrest Weghorst
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-28 23:30:18ID requested
12020-10-16 00:32:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Caspase-3, chicken, neurodevelopment, brainstem
Contact List
Karina Cramer
contact affiliationDepartment of Neurobiology and Behavior, University of California, Irvine
contact emailcramerk@uci.edu
lab head
Forrest Weghorst
contact affiliationDepartment of Neurobiology and Behavior, University of California, Irvine
contact emailfweghors@uci.edu
dataset submitter
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