⮝ Full datasets listing
PXD021722
PXD021722 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Hypoxia Regulates Overall mRNA Homeostasis by Inducing Met1-linked Linear Ubiquitination of AGO2 in Cancer Cells |
| Description | Hypoxia is the most prominent feature in human solid tumors and induces activation of hypoxia-inducible factors and their downstream genes to promote cancer progression. However, whether and how hypoxia regulates overall mRNA homeostasis is unclear. Here we show that hypoxia inhibits global-mRNA decay in cancer cells. Mechanistically, hypoxia induces the interaction of AGO2 with HOIL-1L/HOIP, two crucial components of a linear ubiquitin chain assembly complex, which co-localizes with miRNA-induced silencing complex and in turn catalyzes AGO2 occurring Met1-linked linear ubiquitination (M1-Ubi). A series of biochemical experiments reveal that M1-Ubi of AGO2 restrains miRNA-mediated gene silencing. Moreover, combination analyses of the AGO2-associated mRNA transcriptome by RIP-Seq and the mRNA transcriptome by RNA-Seq confirm that AGO2 M1-Ubi interferes miRNA-targeted mRNA recruiting to AGO2, and thereby facilitates accumulation of global mRNAs. By this mechanism, short-term hypoxia may protect overall mRNAs and enhances stress tolerance, whereas long-term hypoxia in tumor cells results in seriously changing the entire gene expression profile to drive cell malignant evolution. |
| HostingRepository | iProX |
| AnnounceDate | 2021-04-06 |
| AnnouncementXML | Submission_2023-03-01_23:11:24.296.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hailong Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | ubiquitination signature dipeptidyl lysine |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-09-28 20:35:31 | ID requested | |
| ⏵ 1 | 2023-03-01 23:11:26 | announced |
Publication List
Keyword List
| submitter keyword: Hypoxia, AGO2, miRISC, Met1-linked linear ubiquitination, Global mRNA homeostasis, mRNA decay |
Contact List
| Jianxiu Yu | |
|---|---|
| contact affiliation | Shanghai Jiao Tong University school of medicine, Department of Biochemistry and Molecular Cell Biology |
| contact email | Jianxiu.Yu@shsmu.edu.cn |
| lab head | |
| Hailong Zhang | |
| contact affiliation | Shanghai Jiao Tong University school of medicine, Department of Biochemistry and Molecular Cell Biology |
| contact email | zhanghailonghello@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
