<<< Full experiment listing

PXD021712

PXD021712 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHepatitis B Core Protein is Posttranslationally Modified through K29-linked Ubiquitination
DescriptionHepatitis B virus (HBV) core protein (HBc) plays many roles in the HBV life cycle such as regulation of transcription, RNA encapsidation, reverse transcription and viral release. To accomplish these functions, HBc interacts with many host proteins and undergoes different posttranslational modifications (PTMs). One of the most common PTM is ubiquitination that was shown to change function, stability, and intracellular localization of different viral proteins, but the role of HBc ubiquitination in the HBV life cycle remains unknown. Here, we found that HBc protein is posttranslationally modified through K29-linked ubiquitination. We performed a series of co-immunoprecipitation experiments with wild type HBc, lysine to arginine HBc mutants and wild type ubiquitin, single lysine to arginine ubiquitin mutants or single ubiquitin-accepting lysine constructs. We observed that HBc protein could be modified by ubiquitination in transfected as well as infected hepatoma cells. In addition, ubiquitination predominantly occurred on HBc lysine 7 and the preferred ubiquitin chain linkage was through ubiquitin-K29. Mass spectrometry (MS) analyses detected UBR5 as a potential E3 ubiquitin ligase involved in K29-linked ubiquitination. These findings emphasize that ubiquitination of HBc may play an important role in HBV life cycle.
HostingRepositoryPRIDE
AnnounceDate2020-12-01
AnnouncementXMLSubmission_2020-12-01_08:37:37.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD021712
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMartin Hubalek
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-28 06:02:06ID requested
12020-12-01 08:37:38announced
Publication List
Langerová H, Lubyová B, Zábranský A, Hubálek M, Glendová K, Aillot L, Hodek J, Strunin D, Janovec V, Hirsch I, Weber J, Hepatitis B Core Protein Is Post-Translationally Modified through K29-Linked Ubiquitination. Cells, 9(12):(2020) [pubmed]
Keyword List
submitter keyword: Hepatitis B virus
HBc
Posttranslational modifications
Ubiquitination
Ubiquitin
E3 ubiquitin-protein ligase
Contact List
Martin Hubalek
contact affiliationInstitute of Organic Chemistry an Biochmeistry, CAS, Prague, The Czech Republic
contact emailhubalek@uochb.cas.cz
lab head
Martin Hubalek
contact affiliationInstitute of Organic Chemistry and Biochemistry, Czech Academy of Science
contact emailhubalek@uochb.cas.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
[ + ]